Production of autoantibodies associated with polyclonal activation in Yersinia enterocolitica 0:8-infected mice

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRamos, Orivaldo Pereira
dc.creatorCangiani Silva, Eloisa Elena
dc.creatorFalcão, Deise Pasetto
dc.creatorMachado de Medeiros, Beatriz Maria
dc.date2014-05-27T11:21:17Z
dc.date2016-10-25T21:25:25Z
dc.date2014-05-27T11:21:17Z
dc.date2016-10-25T21:25:25Z
dc.date2005-03-29
dc.date.accessioned2017-04-06T09:37:47Z
dc.date.available2017-04-06T09:37:47Z
dc.identifierMicrobiology and Immunology, v. 49, n. 2, p. 129-137, 2005.
dc.identifier0385-5600
dc.identifierhttp://hdl.handle.net/11449/132267
dc.identifierhttp://acervodigital.unesp.br/handle/11449/132267
dc.identifier10.1111/j.1348-0421.2005.tb03712.x
dc.identifierWOS:000227142600005
dc.identifier2-s2.0-14944363055
dc.identifierhttp://dx.doi.org/10.1111/j.1348-0421.2005.tb03712.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/942807
dc.descriptionPolyclonal lymphocyte stimulation is one of the immunomodulatory mechanisms induced by arthritogenic pathogens. In this study we examined the polyclonal activation potential of a virulent strain of Y. enterocolitica serotype O:8 (WA 2707+) and its plasmidless isogenic pair (WA 2707-). SPF Swiss mice were infected intragastrically and spleen cells were obtained on days 7, 14, 21, 28, 35 and 42 after infection. The number of cells secreting nonspecific immunoglobulins of IgG, IgM and IgA isotypes was determined by the ELISPOT technique. The presence of serum-specific antibodies was investigated by ELISA and the presence of autoantibodies by dot-blot assay. Although the patterns of infection of the two bacterial strains were almost the same, only the animals infected with the virulent strain presented clinical anomalies. Neither arthritic nor inflammatory signs were observed in the joints of the infected animals. The greatest activation observed was that of the nonspecific IgM-secreting cells, and their peak of secretion occurred between the 28th and the 42nd day after infection, for both strains of Y. enterocolitica O:8. Only the animals infected with the virulent strain (WA 2707+) produced IgG-specific antibodies in the serum, from the 28th day after infection. The serum of animals infected with either strain showed reactivity to all the autologous constituents tested, mainly on the 28th and 42nd day after infection. It was concluded that infection of mice with either the virulent strain of Y. enterocolitica O:8 or with its plasmidless isogenic pair resulted in the polyclonal activation of the splenic B lymphocytes including some autoreactive clones.
dc.languageeng
dc.publisherCenter Academic Publ Japan
dc.relationMicrobiology and Immunology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAutoantibodies
dc.subjectPolyclonal activation
dc.subjectYersinia enterocolitica
dc.subjectAutoantibody
dc.subjectImmunoglobulin A antibody
dc.subjectImmunoglobulin G antibody
dc.subjectImmunoglobulin M antibody
dc.subjectAnimal cell
dc.subjectAnimal model
dc.subjectAntibody blood level
dc.subjectAntibody production
dc.subjectAntibody specificity
dc.subjectArthritis
dc.subjectBacterial growth
dc.subjectBacterial strain
dc.subjectBacterial virulence
dc.subjectCell count
dc.subjectColony forming unit
dc.subjectControlled study
dc.subjectDot hybridization
dc.subjectEnzyme linked immunosorbent assay
dc.subjectEnzyme linked immunospot assay
dc.subjectFemale
dc.subjectImmunoglobulin production
dc.subjectLymphocyte activation
dc.subjectMouse
dc.subjectNonhuman
dc.subjectPolyclonal activation
dc.subjectSpleen cell
dc.subjectStrain difference
dc.subjectAnimals
dc.subjectAntibodies, Bacterial
dc.subjectFemale
dc.subjectImmunoglobulins
dc.subjectLymphocyte Count
dc.subjectLymphocytes
dc.subjectMice
dc.subjectSpleen
dc.subjectTime Factors
dc.subjectYersinia Infections
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.titleProduction of autoantibodies associated with polyclonal activation in Yersinia enterocolitica 0:8-infected mice
dc.typeOtro


Este ítem pertenece a la siguiente institución