Reduction in cytoplasmic lipid content in bovine embryos cultured in vitro with linoleic acid in semi-defined medium is correlated with increases in cryotolerance

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAccorsi, Mônica F.
dc.creatorLeão, Beatriz Caetano da Silva
dc.creatorRocha-Frigoni, Nathália Alves de Souza
dc.creatorPerri, Silvia Helena Venturoli
dc.creatorMingoti, Gisele Zoccal
dc.date2015-12-07T15:37:43Z
dc.date2016-10-25T21:23:50Z
dc.date2015-12-07T15:37:43Z
dc.date2016-10-25T21:23:50Z
dc.date2015-09-09
dc.date.accessioned2017-04-06T09:31:38Z
dc.date.available2017-04-06T09:31:38Z
dc.identifierZygote (cambridge, England), p. 1-10, 2015.
dc.identifier1469-8730
dc.identifierhttp://hdl.handle.net/11449/131566
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131566
dc.identifier10.1017/S0967199415000428
dc.identifier0000-0002-3059-4458
dc.identifier26350684
dc.identifierhttp://dx.doi.org/10.1017/S0967199415000428
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/942106
dc.descriptionWe examined whether culturing embryos with linoleic acid (LA) in semi-defined medium reduces lipid accumulation and improves cryosurvival after vitrification. Embryos were cultured with LA (100 μM) and a semi-defined medium was used during in vitro culture (IVC), in which the fetal calf serum was substituted by bovine serum albumin (BSA). There was a reduction (P < 0.05) in the embryonic development rate (Control: 25.8% versus LA: 18.5%), but the proposed system was effective in promoting the decrease (P = 0.0130) in the intracellular lipid content (Control: 27.3 ± 0.7 versus LA: 24.6 ± 0.7 arbitrary fluorescence units of embryos stained with the fluorescent dye Nile Red), consequently increasing (P = 0.0490) the embryo survival after 24h of culture post-warming (Control: 50.0% versus LA: 71.7%). The results question the criteria used to evaluate the efficiency of an in vitro production system specifically with relation to the maximum number of blastocysts produced and suggest that might be more appropriate to improve the desired characteristics of embryos generated in accordance with the specific purpose of in vitro embryo production, commercial or scientific. In conclusion, supplying LA to serum-free culture medium was found to adversely affect the rates of embryo development to the blastocyst stage, but significantly reduced embryo lipid accumulation and improved cryopreservation survival.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherCambridge University Press
dc.relationZygote (cambridge, England)
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIn vitro production
dc.subjectBovine embryo
dc.subjectLinoleic acid
dc.subjectLipid
dc.subjectVitrification
dc.titleReduction in cytoplasmic lipid content in bovine embryos cultured in vitro with linoleic acid in semi-defined medium is correlated with increases in cryotolerance
dc.typeOtro


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