Membrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLeão, Beatriz Caetano da Silva
dc.creatorRocha-Frigoni, Nathalia Alves de Souza
dc.creatorCabral, Elaine Cristina
dc.creatorFranco, Marcos Fernando
dc.creatorFerreira, Christina Ramires
dc.creatorEberlin, Marcos Nogueira
dc.creatorFilgueiras, Paulo Roberto
dc.creatorMingoti, Gisele Zoccal
dc.date2015-12-07T15:35:42Z
dc.date2016-10-25T21:23:34Z
dc.date2015-12-07T15:35:42Z
dc.date2016-10-25T21:23:34Z
dc.date2015
dc.date.accessioned2017-04-06T09:30:32Z
dc.date.available2017-04-06T09:30:32Z
dc.identifierZygote (cambridge, England), v. 23, n. 5, p. 732-741, 2015.
dc.identifier1469-8730
dc.identifierhttp://hdl.handle.net/11449/131452
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131452
dc.identifier10.1017/S0967199414000380
dc.identifier0000-0002-3059-4458
dc.identifier25213102
dc.identifierhttp://dx.doi.org/10.1017/S0967199414000380
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941992
dc.descriptionThis study aimed to evaluate the impact of vitrification on membrane lipid profile obtained by mass spectrometry (MS) of in vitro-produced bovine embryos. Matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) has been used to obtain individual embryo membrane lipid profiles. Due to conditions of analysis, mainly membrane lipids, most favorably phosphatidylcholines (PCs) and sphingomyelins (SMs) have been detected. The following ions described by their mass-to-charge ratio (m/z) and respective attribution presented increased relative abundance (1.2-20×) in the vitrified group: 703.5 [SM (16:0) + H]+; 722.5 [PC (40:3) + Na]+; 758.5 [PC (34:2) + H]+; 762.5 [PC (34:0) + H]+; 790.5 [PC (36:0) + H]+ and 810.5 [PC (38:4) + H]+ and/or [PC (36:1) + Na]+. The ion with a m/z 744.5 [PCp (34:1) and/or PCe (34:2)] was 3.4-fold more abundant in the fresh group. Interestingly, ions with m/z 722.5 or 744.5 indicate the presence of lipid species, which are more resistant to enzymatic degradation as they contain fatty acyl residues linked through ether type bonds (alkyl ether or plasmalogens, indicated by the lowercase 'e' and 'p', respectively) to the glycerol structure. The results indicate that cryopreservation impacts the membrane lipid profile, and that these alterations can be properly monitored by MALDI-MS. Membrane lipids can therefore be evaluated by MALDI-MS to monitor the effect of cryopreservation on membrane lipids, and to investigate changes in lipid profile that may reflect the metabolic response to the cryopreservation stress or changes in the environmental conditions.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherCambridge University Press
dc.relationZygote (cambridge, England)
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine embryo
dc.subjectIn vitro production
dc.subjectLipid profile
dc.subjectMass spectrometry
dc.subjectVitrification
dc.titleMembrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos
dc.typeOtro


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