Effectiveness of hyperbaric oxygen for experimental treatment of schistosomiasis mansoni using praziquantel-free and encapsulated into liposomes: Assay in adult worms and oviposition

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFrezza, Tarsila Ferraz
dc.creatorSouza, Ana Luiza Ribeiro de
dc.creatorPrado, César Corat Ribeiro
dc.creatorOliveira, Claudineide Nascimento Fernandes de
dc.creatorGremião, Maria Palmira Daflon
dc.creatorGiorgio, Selma
dc.creatorDolder, Mary Anne Heidi
dc.creatorJoazeiro, Paulo Pinto
dc.creatorAllegretti, Silmara Marques
dc.date2015-12-07T15:34:40Z
dc.date2016-10-25T21:23:25Z
dc.date2015-12-07T15:34:40Z
dc.date2016-10-25T21:23:25Z
dc.date2015
dc.date.accessioned2017-04-06T09:30:00Z
dc.date.available2017-04-06T09:30:00Z
dc.identifierActa Tropica, v. 150, p. 182-189, 2015.
dc.identifier1873-6254
dc.identifierhttp://hdl.handle.net/11449/131388
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131388
dc.identifier10.1016/j.actatropica.2015.07.022
dc.identifier26215128
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2015.07.022
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941928
dc.descriptionThe treatment of schistosomiasis depends on a single drug: praziquantel (PZQ). However, this treatment presents limitations such as low and/or erratic bioavailability that can contribute to cases of tolerance. Improvements to the available drug are urgently needed and studies with a controlled system of drug release, like liposomes, have been gaining prominence. The present study evaluated the activity and synergy between liposomal-praziquantel (lip.PZQ) and hyperbaric oxygen therapy (HBO). Mice received doses of 60 or 100mg/kg PZQ or lip.PZQ, 50 days post-infection, and after the treatment, were exposed to HBO (3 atmosphere absolute - ATA) for 1h. The viability of adult worms and oviposition were analyzed, by necropsy and Kato-Katz examination performed after 15 days of treatment. A concentration of 100mg/kg of lip.PZQ+HBO was more effective (48.0% reduction of worms, 83.3% reduction of eggs/gram of feces) and 100% of the mice had altered of oograms (indicating interruption of oviposition) compared to other treatments and to the Control group (infected and untreated). It is known that PZQ requires participation of the host immune system to complete its antischistosomal activity and that HBO is able to stimulate the immune system. The drug became more available in the body when incorporated into liposomes and, used with HBO, the HBO worked as an adjuvant. This explains the decreases of oviposition and worms recovered form hepatic portal system.
dc.languageeng
dc.publisherElsevier B. V.
dc.relationActa Tropica
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHiperbaric-oxigen
dc.subjectIn vivo assay
dc.subjectLiposomal-praziquantel
dc.subjectPraziquantel
dc.subjectSchistosoma mansoni
dc.titleEffectiveness of hyperbaric oxygen for experimental treatment of schistosomiasis mansoni using praziquantel-free and encapsulated into liposomes: Assay in adult worms and oviposition
dc.typeOtro


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