| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Palacios-Gimenez, O. M. | |
| dc.creator | Marti, D. A. | |
| dc.creator | Cabral-de-Mello, D. C. | |
| dc.date | 2015-12-07T15:33:04Z | |
| dc.date | 2016-10-25T21:23:05Z | |
| dc.date | 2015-12-07T15:33:04Z | |
| dc.date | 2016-10-25T21:23:05Z | |
| dc.date | 2015 | |
| dc.date.accessioned | 2017-04-06T09:28:46Z | |
| dc.date.available | 2017-04-06T09:28:46Z | |
| dc.identifier | Chromosoma, v. 124, n. 3, p. 353-365, 2015. | |
| dc.identifier | 1432-0886 | |
| dc.identifier | http://hdl.handle.net/11449/131252 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/131252 | |
| dc.identifier | 10.1007/s00412-015-0505-1 | |
| dc.identifier | 25605041 | |
| dc.identifier | http://dx.doi.org/10.1007/s00412-015-0505-1 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/941792 | |
| dc.description | Sex chromosomes have evolved many times from morphologically identical autosome pairs, most often presenting several recombination suppression events, followed by accumulation of repetitive DNA sequences. In Orthoptera, most species have an X0♂ sex chromosome system. However, in the subfamily Melanoplinae, derived variants of neo-sex chromosomes (neo-XY♂ or neo-X1X2Y♂) emerged several times. Here, we examined the differentiation of neo-sex chromosomes in a Melanoplinae species with a neo-XY♂/XX♀ system, Ronderosia bergi, using several approaches: (i) classical cytogenetic analysis, (ii) mapping via fluorescent in situ hybridization of some selected repetitive DNA sequences and microdissected sex chromosomes, and (iii) immunolocalization of distinct histone modifications. The microdissected sex chromosomes were also used as sources for Polymerase chain reaction (PCR) amplification of RNA-coding multigene families, to study variants related to the sex chromosomes. Our data suggest that the R. bergi neo-Y has become differentiated after its formation by a Robertsonian translocation and inversions, and has accumulated repetitive DNA sequences. Interestingly, the ex autosomes incorporated into the neo-sex chromosomes retain some autosomal post-translational histone modifications, at least in metaphase I, suggesting that the establishment of functional modifications in neo-sex chromosomes is slower than their sequence differentiation. | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.description | Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.language | eng | |
| dc.publisher | Springer-Verlag Berlin Heidelberg | |
| dc.relation | Chromosoma | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | Rb-translocation | |
| dc.subject | Neo-sex chromosomes | |
| dc.subject | FISH | |
| dc.subject | Repetitive DNA | |
| dc.subject | Histone modification | |
| dc.title | Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers | |
| dc.type | Otro | |
