Brazilian green propolis induced apoptosis in human lung cancer A549 cells through mitochondrial-mediated pathway

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFrión-Herrera, Yahima
dc.creatorDíaz-García, Alexis
dc.creatorRuiz-Fuentes, Jenny
dc.creatorRodríguez-Sánchez, Hermis
dc.creatorSforcin, José Maurício
dc.date2015-12-07T15:32:53Z
dc.date2016-10-25T21:23:02Z
dc.date2015-12-07T15:32:53Z
dc.date2016-10-25T21:23:02Z
dc.date2015
dc.date.accessioned2017-04-06T09:28:36Z
dc.date.available2017-04-06T09:28:36Z
dc.identifierThe Journal Of Pharmacy And Pharmacology, v. 67, n. 10, p. 1448-1456, 2015.
dc.identifier2042-7158
dc.identifierhttp://hdl.handle.net/11449/131233
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131233
dc.identifier10.1111/jphp.12449
dc.identifier26206395
dc.identifierhttp://dx.doi.org/10.1111/jphp.12449
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941773
dc.descriptionPropolis effect on the growth and apoptosis of human lung adenocarcinoma (A549 cells) was investigated as well as its mechanisms. Cells were incubated with propolis for 72 h, and 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were employed to assess cell viability and the inhibitory concentration (IC). Apoptosis was detected by Acridine Orange/Ethidium Bromide and 4',6-diamidino-2-phenylindole staining after 24 and 48 h of incubation with ¼ IC50 of propolis by testing the mitochondrial membrane potential (ΔΨm) and the expression of apoptosis-related genes (p53, Caspase-3, Bax, Bcl-2, Bcl-XL , Noxa, Puma and p21) by reverse transcription polymerase chain reaction. Propolis displayed antiproliferative and cytotoxic effects on A549 cells in a dose- and time-dependent manner, but it did not suppress the growth of normal Vero cells. An enhanced apoptosis was seen in A549 propolis-treated cells after 48 h compared with the control cells. Propolis decreased mitochondrial membrane potential by overexpression of pro-apoptotic genes (Bax and Noxa) and reduction of the antiapoptotic gene Bcl-XL . The expression level of other genes remained unchanged (p53, Caspse-3 and Bax), whereas p21 expression was increased. Propolis induced caspase-independent apoptosis through a p53-independent mitochondrial pathway, and cell cycle arrest by upregulation of p21. Although propolis induces apoptosis mainly by p53-independent manner, it may be induced by another pathway, and new insights may arise for preventing or treating lung cancer.
dc.languageeng
dc.relationThe Journal Of Pharmacy And Pharmacology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectLung cancer
dc.subjectMitochondria pathway
dc.subjectPropolis
dc.titleBrazilian green propolis induced apoptosis in human lung cancer A549 cells through mitochondrial-mediated pathway
dc.typeOtro


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