Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPinheiro, Luiza
dc.creatorBrito, Carla Ivo
dc.creatorOliveira, Adilson de
dc.creatorMartins, Patrícia Yoshida Faccioli
dc.creatorPereira, Valéria Cataneli
dc.creatorCunha, Maria de Lourdes Ribeiro de Souza da
dc.date2015-12-07T15:32:51Z
dc.date2016-10-25T21:23:02Z
dc.date2015-12-07T15:32:51Z
dc.date2016-10-25T21:23:02Z
dc.date2015
dc.date.accessioned2017-04-06T09:28:34Z
dc.date.available2017-04-06T09:28:34Z
dc.identifierToxins, v. 7, n. 9, p. 3688-3699, 2015.
dc.identifier2072-6651
dc.identifierhttp://hdl.handle.net/11449/131230
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131230
dc.identifier10.3390/toxins7093688
dc.identifierPMC4591658.pdf
dc.identifier26389954
dc.identifierPMC4591658
dc.identifierhttp://dx.doi.org/10.3390/toxins7093688
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941770
dc.descriptionAlthough opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.relationToxins
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectStaphylococcus epidermidis
dc.subjectStaphylococcus haemolyticus
dc.subjectCytotoxins
dc.subjectEnterotoxins
dc.titleStaphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
dc.typeOtro


Este ítem pertenece a la siguiente institución