Biological characterization of the Amazon coral Micrurus spixii snake venom: isolation of a new neurotoxic phospholipase A2

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTerra, Angelo Laurence Covatti
dc.creatorMoreira-Dill, Leandro Soares
dc.creatorSimões-Silva, Rodrigo
dc.creatorMonteiro, José Roniele do Nascimento
dc.creatorCavalcante, Walter Luís Garrido
dc.creatorGallacci, Márcia
dc.creatorBarros, Neuza Biguinati
dc.creatorNicolete, Roberto
dc.creatorTeles, Carolina Bioni Garcia
dc.creatorMedeiros, Patrícia Soares Maria de
dc.creatorZanchi, Fernando Berton
dc.creatorZuliani, Juliana Pavan
dc.creatorCalderon, Leonardo de Azevedo
dc.creatorStábeli, Rodrigo Guerino
dc.creatorSoares, Andreimar Martins
dc.date2015-12-07T15:32:47Z
dc.date2016-10-25T21:23:01Z
dc.date2015-12-07T15:32:47Z
dc.date2016-10-25T21:23:01Z
dc.date2015
dc.date.accessioned2017-04-06T09:28:31Z
dc.date.available2017-04-06T09:28:31Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology, v. 103, p. 1-11, 2015.
dc.identifier1879-3150
dc.identifierhttp://hdl.handle.net/11449/131223
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131223
dc.identifier10.1016/j.toxicon.2015.06.011
dc.identifier26095535
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2015.06.011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941763
dc.descriptionThe Micrurus genus is the American representative of Elapidae family. Micrurus spixii is endemic of South America and northern states of Brazil. Elapidic venoms contain neurotoxins that promote curare-mimetic neuromuscular blockage. In this study, biochemical and functional characterizations of M. spixii crude venom were performed and a new neurotoxic phospholipase A2 called MsPLA2-I was isolated. M. spixii crude venom caused severe swelling in the legs of tested mice and significant release of creatine kinase (CK) showing its myotoxic activity. Leishmanicidal activity against Leishmania amazonensis (IC50 1.24 μg/mL) was also observed, along with antiplasmodial activity against Plasmodium falciparum, which are unprecedented for Micrurus venoms. MsPLA2-I with a Mr 12,809.4 Da was isolated from the crude venom of M. spixii. The N-terminal sequencing of a fragment of 60 amino acids showed 80% similarity with another PLA2 from Micrurus altirostris. This toxin and the crude venom showed phospholipase activity. In a mouse phrenic nerve-diaphragm preparation, M. spixii venom and MsPLA2-I induced the blockage of both direct and indirect twitches. While the venom presented a pronounced myotoxic activity, MsPLA2-I expressed a summation of neurotoxic activity. The results of this study make M. spixii crude venom promising compounds in the exploration of molecules with microbicidal potential.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoodernação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherElsevier B. V.
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMicrobicidal potential
dc.subjectMicrurus spixii
dc.subjectNeurotoxicity
dc.subjectPhospholipase a(2)
dc.subjectSnake venom
dc.titleBiological characterization of the Amazon coral Micrurus spixii snake venom: isolation of a new neurotoxic phospholipase A2
dc.typeOtro


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