HLA-E coding and 3' untranslated region variability determined by next-generation sequencing in two West-African population samples

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCastelli, Erick C.
dc.creatorMendes-Junior, Celso T.
dc.creatorSabbagh, Audrey
dc.creatorPorto, Iane O. P.
dc.creatorGarcia, André
dc.creatorRamalho, Jaqueline
dc.creatorLima, Thálitta H. A.
dc.creatorMassaro, Juliana D.
dc.creatorDias, Fabrício C.
dc.creatorCollares, Cristhianna V. A.
dc.creatorJamonneau, Vincent
dc.creatorBucheton, Bruno
dc.creatorCamara, Mamadou
dc.creatorDonadi, Eduardo A.
dc.date2015-12-07T15:31:43Z
dc.date2016-10-25T21:22:46Z
dc.date2015-12-07T15:31:43Z
dc.date2016-10-25T21:22:46Z
dc.date2015
dc.date.accessioned2017-04-06T09:27:34Z
dc.date.available2017-04-06T09:27:34Z
dc.identifierHuman Immunology, 2015.
dc.identifier1879-1166
dc.identifierhttp://hdl.handle.net/11449/131114
dc.identifierhttp://acervodigital.unesp.br/handle/11449/131114
dc.identifier10.1016/j.humimm.2015.06.016
dc.identifier26187162
dc.identifierhttp://dx.doi.org/10.1016/j.humimm.2015.06.016
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/941654
dc.descriptionHLA-E is a non-classical Human Leucocyte Antigen class I gene with immunomodulatory properties. Whereas HLA-E expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with NK cells and T lymphocytes, being important for immunosurveillance and also for fighting against infections. HLA-E is usually the most conserved locus among all class I genes. However, most of the previous studies evaluating HLA-E variability sequenced only a few exons or genotyped known polymorphisms. Here we report a strategy to evaluate HLA-E variability by next-generation sequencing (NGS) that might be used to other HLA loci and present the HLA-E haplotype diversity considering the segment encoding the entire HLA-E mRNA (including 5'UTR, introns and the 3'UTR) in two African population samples, Susu from Guinea-Conakry and Lobi from Burkina Faso. Our results indicate that (a) the HLA-E gene is indeed conserved, encoding mainly two different protein molecules; (b) Africans do present several unknown HLA-E alleles presenting synonymous mutations; (c) the HLA-E 3'UTR is quite polymorphic and (d) haplotypes in the HLA-E 3'UTR are in close association with HLA-E coding alleles. NGS has proved to be an important tool on data generation for future studies evaluating variability in non-classical MHC genes.
dc.languageeng
dc.publisherElsevier B. V.
dc.relationHuman Immunology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAfrica
dc.subjectHla-e
dc.subjectHaplotypes
dc.subjectNext-generation sequencing (ngs)
dc.subjectNon-classical hla
dc.subjectPolymorphisms
dc.subjectWest-african populations
dc.titleHLA-E coding and 3' untranslated region variability determined by next-generation sequencing in two West-African population samples
dc.typeOtro


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