Otro
Caracterização química dos géis produzidos pelas bactérias diazotróficas Rhizobium tropici e Mesorhizobium sp.
Registration in:
Quimica Nova. São Paulo: Soc Brasileira Quimica, v. 35, n. 4, p. 705-708, 2012.
0100-4042
10.1590/S0100-40422012000400009
WOS:000304226400009
S0100-40422012000400009.pdf
Author
Monteiro, Nilson Kobori
Aranda-Selverio, Gabriel
Exposti, Diego Tadeu Degli
Silva, Maria de Lourdes Corradi da
Lemos, Eliana Gertrudes Macedo
Campanharo, João Carlos
Silveira, Joana Léa Meira
Abstract
The exopolysaccharides with characteristics of gel produced by Rhizobium tropici (EPS RT) and Mesorhizobium sp (EPS MR) are acidic heteropolysaccharide composed mainly of glucose and galactose in a molar ratio of 4:1 and 5:1 respectively, with traces of mannose (~ 1%). Chemical analysis showed the presence of uronic acid, pyruvate and acetyl-substituents in the structures of both polymers. Experiments of gel permeation chromatography and polyacrylamide gel electrophoresis showed that EPS RT and EPS MR are homogeneous molecules with low grade of polydispersity. The EPS were characterized using spectroscopic techniques of FT-IR, ¹H and 13C-NMR. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)