Determination of estrogenic mycotoxins in environmental water samples by low-toxicity dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorEmidio, Elissandro Soares
dc.creatorSilva, Claudia Pereira da
dc.creatorRodrigues de Marchi, Mary Rosa
dc.date2015-10-21T20:22:31Z
dc.date2016-10-25T21:08:22Z
dc.date2015-10-21T20:22:31Z
dc.date2016-10-25T21:08:22Z
dc.date2015-04-24
dc.date.accessioned2017-04-06T09:07:00Z
dc.date.available2017-04-06T09:07:00Z
dc.identifierJournal Of Chromatography A. Amsterdam: Elsevier Science Bv, v. 1391, p. 1-8, 2015.
dc.identifier0021-9673
dc.identifierhttp://hdl.handle.net/11449/129105
dc.identifierhttp://acervodigital.unesp.br/handle/11449/129105
dc.identifierhttp://dx.doi.org/10.1016/j.chroma.2015.02.067
dc.identifierWOS:000353427000001
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0021967315003404
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/939660
dc.descriptionA novel, simple, rapid and eco-friendly method based on dispersive liquid-liquid microextraction using a bromosolvent was developed to determine six estrogenic mycotoxins (zearalenone, zearalanone, alpha-zearalanol, beta-zearalanol, alpha-zearalenol and beta-zearalenol) in water samples by liquid chromatography-electrospray ionization tandem mass spectrometry in the negative mode (LC-ESI-MS/MS). The optimal conditions for this method include the use of 100 mu L bromocyclohexane as an extraction solvent (using a non-dispersion solvent), 10 mL of aqueous sample (adjusted to pH 4), a vortex extraction time of 2 min, centrifugation for 10 min at 3500 rpm and no ionic strength adjustment. The calibration function was linear and was verified by applying the Mandel fitting test with a 95% confidence level. No matrix effect was observed. According to the relative standard deviations (RSDs), the precision was better than 13% for the repeatability and intermediate precision. The average recoveries of the spiked compounds ranged from 81 to 118%. The method limits of detection (LOD) and quantification (LOQ) considering a 125-fold pre-concentration step were 4-20 and 8-40 ng L-1, respectively. Next, the method was applied to the analysis of the environmental aqueous samples, demonstrating the presence of beta-zearalanol and zearalanone in the river water samples. (C) 2015 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationJournal Of Chromatography A
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEstrogenic mycotoxins
dc.subjectBromosolvents
dc.subjectDispersive liquid-liquid microextraction
dc.subjectWater samples
dc.subjectLC-MS/MS
dc.titleDetermination of estrogenic mycotoxins in environmental water samples by low-toxicity dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry
dc.typeOtro


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