Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMoraes, Karen C. M.
dc.creatorDiniz, Livia F.
dc.creatorBahia, Maria Terezinha
dc.date2015-10-21T13:12:12Z
dc.date2016-10-25T21:00:12Z
dc.date2015-10-21T13:12:12Z
dc.date2016-10-25T21:00:12Z
dc.date2015-04-01
dc.date.accessioned2017-04-06T09:00:19Z
dc.date.available2017-04-06T09:00:19Z
dc.identifierMemorias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 181-191, 2015.
dc.identifier0074-0276
dc.identifierhttp://hdl.handle.net/11449/128678
dc.identifierhttp://acervodigital.unesp.br/handle/11449/128678
dc.identifierhttp://dx.doi.org/10.1590/0074-02760140311
dc.identifierS0074-02762015000200181
dc.identifierWOS:000354066400004
dc.identifierS0074-02762015000200181.pdf
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200181&lng=en&nrm=iso&tlng=en
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/939234
dc.descriptionChagas disease, caused by the intracellular protozoan Trypanosoma cruzi, is a serious health problem in Latin America. During this parasitic infection, the heart is one of the major organs affected. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. When cells are infected with T. cruzi, they develop an inflammatory response, in which cyclooxygenase-2 (COX-2) catalyses rate-limiting steps in the arachidonic acid pathway. However, how the parasite interaction modulates COX-2 activity is poorly understood. In this study, the H9c2 cell line was used as our model and we investigated cellular and biochemical aspects during the initial 48 h of parasitic infection. Oscillatory activity of COX-2 was observed, which correlated with the control of the pro-inflammatory environment in infected cells. Interestingly, subcellular trafficking was also verified, correlated with the control of Cox-2 mRNA or the activated COX-2 protein in cells, which is directly connected with the assemble of stress granules structures. Our collective findings suggest that in the very early stage of the T. cruzi-host cell interaction, the parasite is able to modulate the cellular metabolism in order to survives.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherFundaco Oswaldo Cruz
dc.relationMemorias do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCardiac cells
dc.subjectChagas disease
dc.subjectEnzyme activity
dc.subjectFluorescence microscopy
dc.subjectPro-inflammatory process
dc.subjectSubcellular trafficking
dc.titleRole of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction
dc.typeOtro


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