Effects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSouza Rocha-Frigoni, Nathalia Alves de
dc.creatorSilva Leao, Beatriz Caetano da
dc.creatorNogueira, Eriklis
dc.creatorAccorsi, Monica Ferreira
dc.creatorMingoti, Gisele Zoccal
dc.date2015-10-21T13:10:07Z
dc.date2016-10-25T20:59:40Z
dc.date2015-10-21T13:10:07Z
dc.date2016-10-25T20:59:40Z
dc.date2015-04-01
dc.date.accessioned2017-04-06T08:58:34Z
dc.date.available2017-04-06T08:58:34Z
dc.identifierZygote. New York: Cambridge Univ Press, v. 23, n. 2, p. 159-168, 2015.
dc.identifier0967-1994
dc.identifierhttp://hdl.handle.net/11449/128467
dc.identifierhttp://acervodigital.unesp.br/handle/11449/128467
dc.identifierhttp://dx.doi.org/10.1017/S0967199413000361
dc.identifierWOS:000352708100001
dc.identifier0000-0002-3059-4458
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/939023
dc.descriptionThis study examined the effects of antioxidant supplementation and O-2 tension on embryo development, cryotolerance and intracellular reactive oxygen species (ROS) levels. The antioxidant supplementation consisted of 0.6 mM cysteine (CYST); 0.6 mM cysteine + 100 mu M cysteamine (C+C); 100 IU catalase (CAT) or 100 mu M beta-mercaptoethanol (beta-ME) for 3 or 7 days of in vitro culture (IVC). Two O-2 tensions (20% O-2 [5% CO2 in air] or 7% O-2, 5% CO2 and 88% N-2 [gaseous mixture]) were examined. After 7 days of antioxidant supplementation, the blastocyst frequencies were adversely affected (P<0.05) by CYST (11.2%) and C+C (1.44%), as well as by low O-2 tension (17.2% and 11.11% for 20% and 7% O-2, respectively) compared with the control (26.6%). The blastocyst re-expansion rates were not affected (P > 0.05) by the treatments (range, 66-100%). After 3 days of antioxidant supplementation, the blastocyst frequencies were not affected (P > 0.05) by any of the antioxidants (range, 43.6-48.5%), but they were reduced by low O-2 tension (P<0.05) (52.1% and 38.4% for 20% and 7% O-2, respectively). The intracellular ROS levels, demonstrated as arbitrary fluorescence units, were not affected (P > 0.05) by antioxidant treatment (range, 0.78 to 0.95) or by O-2 tension (0.86 and 0.88 for 20% and 7% O-2, respectively). The re-expansion rates were not affected (P > 0.05) by any of the treatments (range, 63.6-93.3%). In conclusion, intracellular antioxidant supplementation and low O-2 tension throughout the entire IVC period were deleterious to embryo development. However, antioxidant supplementation up to day 3 of IVC did not affect the blastocyst frequencies or intracellular ROS levels.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherCambridge Univ Press
dc.relationZygote
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntioxidants
dc.subjectCryotolerance
dc.subjectEmbryo culture
dc.subjectEmbryo development
dc.subjectGaseous atmosphere
dc.titleEffects of gaseous atmosphere and antioxidants on the development and cryotolerance of bovine embryos at different periods of in vitro culture
dc.typeOtro


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