Meiotic arrest of sheep oocytes using roscovitine under different medium compositions

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCrocomo, Leticia Ferrari
dc.creatorVela Ulian, Carla Maria
dc.creatorBranchini, Naiana da Silva
dc.creatorSilva, Denise Theodoro da
dc.creatorMarques Filho, Wolff Camargo
dc.creatorLandim Alvarenga, Fernanda da Cruz
dc.creatorBicudo, Sony Dimas
dc.date2015-10-21T13:09:59Z
dc.date2016-10-25T20:59:39Z
dc.date2015-10-21T13:09:59Z
dc.date2016-10-25T20:59:39Z
dc.date2015-05-01
dc.date.accessioned2017-04-06T08:58:28Z
dc.date.available2017-04-06T08:58:28Z
dc.identifierSmall Ruminant Research. Amsterdam: Elsevier Science Bv, v. 126, p. 52-58, 2015.
dc.identifier0921-4488
dc.identifierhttp://hdl.handle.net/11449/128454
dc.identifierhttp://acervodigital.unesp.br/handle/11449/128454
dc.identifierhttp://dx.doi.org/10.1016/j.smallrumres.2015.02.022
dc.identifierWOS:000355041500009
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0921448815001042
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/939010
dc.descriptionAttempts to maintain the meiotic arrest under suitable culture conditions have been performed in order to improve the quality of oocytes in vitro matured. So, our study aimed to evaluate the potential of roscovitine, an inhibitor of cyclin-dependent kinases, to reversibly arrest the meiosis of sheep oocytes in medium supplemented with gonadotropins and/or serum. Cumulus-oocyte complexes (COCs) were treated, for 20 h, with 75 mu M roscovitine (Rosco) in basic maturation medium (Basic) containing 10% fetal bovine serum (FBS) and 0.1 IU/mL FSH plus 0.1 IU/mL LH (Enriched). After this, COCs were in vitro matured (IVM) for a further 18 h in the presence of gonadotropins and serum. At 20 and 38 h of culture, cumulus expansion and nuclear maturation were assessed under stereomicroscope and by Hoechst 33342 staining, respectively. Our findings demonstrate that total cumulus expansion occurred only in medium containing gonadotropins and serum. In the presence of roscovitine, however, the cumulus expansion was inhibited in a not fully reversible manner, independently of medium composition. The GV rate in the Rosco FBS group (64.6%) was significantly higher than that of Rosco Enriched (51.8%); however, both did not differ from that observed in the Rosco Basic (56.2%). In all roscovitine treatments, the meiotic arrest was reversible after the additional culture for 18 h in inhibitor-free medium. Besides, significant proportion of oocytes from Basic, FBS and Enriched groups reached the MII stage after 20 and 38 h of culture. Therefore, we can infer that (i) gonadotropins and serum did not affect the reversible meiotic arrest promoted by roscovitine in sheep oocytes; (ii) the maximum efficiency of meiosis inhibition occurred in serum-supplemented medium; (iii) roscovitine action on cumulus cells was not fully reversible independently of medium composition.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationSmall Ruminant Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectInhibitor
dc.subjectNuclear maturation
dc.subjectCumulus expansion
dc.subjectGonadotropin
dc.subjectSerum
dc.subjectOvine
dc.titleMeiotic arrest of sheep oocytes using roscovitine under different medium compositions
dc.typeOtro


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