Gene and protein expression of oestrogen-beta and progesterone receptors in facial melasma and adjacent healthy skin in women

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTamega, A. de A.
dc.creatorMiot, H. A.
dc.creatorMoco, N. P.
dc.creatorSilva, M. G.
dc.creatorMarques, M. E. A.
dc.creatorMiot, L. D. B.
dc.date2015-10-21T13:08:58Z
dc.date2016-10-25T20:59:19Z
dc.date2015-10-21T13:08:58Z
dc.date2016-10-25T20:59:19Z
dc.date2015-04-01
dc.date.accessioned2017-04-06T08:57:19Z
dc.date.available2017-04-06T08:57:19Z
dc.identifierInternational Journal Of Cosmetic Science, v. 37, n. 2, p. 222-228, 2015.
dc.identifier0142-5463
dc.identifierhttp://hdl.handle.net/11449/128322
dc.identifierhttp://acervodigital.unesp.br/handle/11449/128322
dc.identifierhttp://dx.doi.org/10.1111/ics.12186
dc.identifierWOS:000350547600008
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/ics.12186/abstract?systemMessage=Wiley+Online+Library+and+related+systems+will+have+3+hours+of+downtime+on+Saturday+12th+September+2015+from+10%3A00-13%3A00+BST+%2F+05%3A00-08%3A00+EDT+%2F+17%3A00-20%3A00+SGT+for+essential+maintenance.++Apologies+for+the+inconvenience.
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/938878
dc.descriptionSynopsisObjectiveCompare gene and protein expression for oestrogen receptor- (ER-) and progesterone receptor (PR) in facial melasma and adjacent healthy skin.MethodsA cross-sectional study including 42 women with facial melasma, conducted at the Dermatology Service of Botucatu Medical School of SAo Paulo State University, Brazil. Biopsies of the melasma skin were performed, together with healthy surrounding skin. The gene expression (real-time PCR) of the hormone receptors in the tissue was evaluated. Subsequently, skin fragments were immunostained for nuclear ER- and PR, evaluated according to their HSCORE (epidermis) and percentage of staining per microscopic field (dermis).ResultsMessenger RNA tissue expression for ER- and PR showed no difference between melasma-affected skin fragments and the healthy perilesional areas (P>0.2). Median nuclear epithelial expression for ER- and PR was higher in lesioned skin (HSCORE 157 and 58) than in the healthy perilesional skin (HSCORE 97 and 19; P<0.01), with no difference in dermal immunostaining. Nuclear histological expression for ER- was associated to sun-induced melasma and negative familiar background; PR expression was associated to sun-induced melasma and darker phototypes.ConclusionNo difference was observed in gene expression for oestrogen- and progesterone receptors in melasma-affected skin compared with adjacent healthy skin. However, the higher protein expression of these receptors in melasma-affected epithelia suggests hormonal participation in the pathogenesis of this disease.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationInternational Journal Of Cosmetic Science
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectimmunohistochemistry
dc.subjectmelanin
dc.subjectmelanocytes
dc.subjectmelanogenesis
dc.subjectmelasma
dc.subjectoestrogen receptor-
dc.subjectpigmentation
dc.subjectprogesterone receptor
dc.titleGene and protein expression of oestrogen-beta and progesterone receptors in facial melasma and adjacent healthy skin in women
dc.typeOtro


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