Combining xanthan and chitosan membranes to multipotent mesenchymal stromal cells as bioactive dressings for dermo-epidermal wounds

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBellini, M. Z.
dc.creatorCaliari Oliveira, C.
dc.creatorMizukami, A.
dc.creatorSwiech, K.
dc.creatorCovas, D. T.
dc.creatorDonadi, E. A.
dc.creatorOliveira Neto, Pedro de
dc.creatorMoraes, A. M.
dc.date2015-08-21T17:53:27Z
dc.date2016-10-25T20:56:12Z
dc.date2015-08-21T17:53:27Z
dc.date2016-10-25T20:56:12Z
dc.date2014
dc.date.accessioned2017-04-06T08:45:42Z
dc.date.available2017-04-06T08:45:42Z
dc.identifierJournal of Biomaterials Applications, v. 29, n. 8, , p. 1155-1166, 2014.
dc.identifier0885-3282
dc.identifierhttp://hdl.handle.net/11449/126909
dc.identifierhttp://acervodigital.unesp.br/handle/11449/126909
dc.identifierhttp://dx.doi.org/10.1177/0885328214553959
dc.identifier4638952263502744
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25281644
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/937481
dc.descriptionThe association between tridimensional scaffolds to cells of interest has provided excellent perspectives for obtaining viable complex tissues in vitro, such as skin, resulting in impressive advances in the field of tissue engineering applied to regenerative therapies. The use of multipotent mesenchymal stromal cells in the treatment of dermo-epidermal wounds is particularly promising due to several relevant properties of these cells, such as high capacity of proliferation in culture, potential of differentiation in multiple skin cell types, important paracrine and immunomodulatory effects, among others. Membranes of chitosan complexed with xanthan may be potentially useful as scaffolds for multipotent mesenchymal stromal cells, given that they present suitable physico-chemical characteristics and have adequate tridimensional structure for the adhesion, growth, and maintenance of cell function. Therefore, the purpose of this work was to assess the applicability of bioactive dressings associating dense and porous chitosan-xanthan membranes to multipotent mesenchymal stromal cells for the treatment of skin wounds. The membranes showed to be non-mutagenic and allowed efficient adhesion and proliferation of the mesenchymal stromal cells in vitro. In vivo assays performed with mesenchymal stromal cells grown on the surface of the dense membranes showed acceleration of wound healing in Wistar rats, thus indicating that the use of this cell-scaffold association for tissue engineering purposes is feasible and attractive.
dc.languageeng
dc.relationJournal of Biomaterials Applications
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectScaffolds
dc.subjectChitosan
dc.subjectDressings
dc.subjectRegenerative medicine
dc.subjectSkin lesions
dc.subjectStem cells
dc.subjectXanthan gum
dc.titleCombining xanthan and chitosan membranes to multipotent mesenchymal stromal cells as bioactive dressings for dermo-epidermal wounds
dc.typeOtro


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