Antimicrobial photodynamic therapy against pathogenic bacterial suspensions and biofilms using chloro-aluminum phthalocyanine encapsulated in nanoemulsions

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRibeiro, Ana Paula Dias
dc.creatorAndrade, Mariana Carvalho
dc.creatorBagnato, Vanderlei Salvador
dc.creatorVergani, Carlos Eduardo
dc.creatorPrimo, Fernando Lucas
dc.creatorTedesco, Antônio Cláudio
dc.creatorPavarina, Ana Claudia
dc.date2015-08-06T16:13:08Z
dc.date2016-10-25T20:53:42Z
dc.date2015-08-06T16:13:08Z
dc.date2016-10-25T20:53:42Z
dc.date2013
dc.date.accessioned2017-04-06T08:36:27Z
dc.date.available2017-04-06T08:36:27Z
dc.identifierLasers in Medical Science, v. 30, n. 2, p. 549-559, 2013.
dc.identifier0268-8921
dc.identifierhttp://hdl.handle.net/11449/125797
dc.identifierhttp://acervodigital.unesp.br/handle/11449/125797
dc.identifierhttp://dx.doi.org/10.1007/s10103-013-1354-x
dc.identifier8867670539105403
dc.identifier1531215699841687
dc.identifier4947860249518663
dc.identifier3003130522427820
dc.identifier5809961609784365
dc.identifierhttp://link.springer.com/article/10.1007%2Fs10103-013-1354-x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/936389
dc.descriptionAntimicrobial photodynamic therapy represents an alternative method of killing resistant pathogens. Efforts have been made to develop delivery systems for hydrophobic drugs to improve the photokilling. This study evaluated the photodynamic effect of chloro-aluminum phthalocyanine (ClAlPc) encapsulated in nanoemulsions (NE) on methicillin-susceptible and methicillin-resistant Staphylococcus aureus suspensions and biofilms. Suspensions and biofilms were treated with different delivery systems containing ClAlPc. After the pre-incubation period, the drug was washed-out and irradiation was performed with LED source (660 ± 3 nm). Negative control samples were not exposed to ClAlPc or light. For the suspensions, colonies were counted (colony-forming units per milliliter (CFU/mL)). The metabolic activity of S. aureus suspensions and biofilms were evaluated by the XTT assay. The efficiency was dependent on the delivery system, superficial load and light dose. Cationic NE-ClAlPc and free-ClAlPc caused photokilling of the both strains of S. aureus. For biofilms, cationic NE-ClAlPc reduced cell metabolism by 80 and 73 % of susceptible and resistant strains, respectively. Although anionic NE-ClAlPc caused a significant CFU/ml reduction for MSSA and MRSA, it was not capable of reducing MRSA biofilm metabolism. This therapy may represent an alternative treatment for eradicating resistant strains.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationLasers in Medical Science
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAntimicrobial photodynamic therapy
dc.subjectDrug delivery systems
dc.subjectPhthalocyanine
dc.subjectMRSA
dc.titleAntimicrobial photodynamic therapy against pathogenic bacterial suspensions and biofilms using chloro-aluminum phthalocyanine encapsulated in nanoemulsions
dc.typeOtro


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