Heparin and dextran sulfate: their role on rsv infectivity

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRubio, Marcelo
dc.creatorToledo, Karina Alves
dc.creatorCalça, Jaqueline
dc.creatorBonfim, Caroline Measso
dc.creatorGomes, Deriane Elias
dc.creatorDurigon, Edison Luiz
dc.creatorFossey, Marcelo Andres
dc.creatorRahal, Paula
dc.creatorSouza, Fátima Pereira de
dc.date2015-04-27T11:56:06Z
dc.date2016-10-25T20:47:12Z
dc.date2015-04-27T11:56:06Z
dc.date2016-10-25T20:47:12Z
dc.date2010
dc.date.accessioned2017-04-06T08:10:52Z
dc.date.available2017-04-06T08:10:52Z
dc.identifierVirus Reviews and Research, v. 15, n. 02, p. 92-107, 2010.
dc.identifier1519-2563
dc.identifierhttp://hdl.handle.net/11449/122863
dc.identifierhttp://acervodigital.unesp.br/handle/11449/122863
dc.identifier3313511334783986
dc.identifier7960752099426224
dc.identifier6069686231701579
dc.identifier0942014519604427
dc.identifier4101562077663619
dc.identifier7991082362671212
dc.identifierhttp://www.sbv.org.br/vrr/ISSUE15-2.html
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/933481
dc.descriptionRespiratory syncytial virus is the major cause of acute lower respiratory tract illness in infants and young children. Because there is currently no licensed vaccine for RSV, there is a substantial interest in the identification and development of RSV specific inhibitory agents. There are clinical evidences that glycosaminoglycans (GAGs) are potential inhibitors of viral infection. In this study, the performance of two GAGs (heparin and dextran sulfate) were compared for their antiviral and virucidal activities on RSV. Analysis was performed using an in vitro infection model where, previously to infection, Hep-2 cells or RSV were incubated with heparin or dextran sulfate. The presence of viral particles was analyzed by Reverse Transcriptase-Polimerase Chain Reaction (RT-PCR) and indirect immunofluorescence assays (IFA). The results showed that viral infection was more efficiently inhibited when Hep-2 cells were pre-incubated with heparin or, when viral particles were pre-incubated with dextran sulfate. Our study suggest that, in the absence of cellular death, heparin and dextran sulfate reduce RSV infection by different mechanisms, antiviral and virucidal ones, respectively. These data contribute for recent medical, microbiology and biochemical studies which suggest that the use of antiviral and virucidal compounds as more effective treatment to control virus infections.
dc.languageeng
dc.relationVirus Reviews and Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectantiviral
dc.subjectvirucidal
dc.subjecthuman syncytial respiratory virus
dc.subjectheparin
dc.subjectdextran
dc.subjectsulfate
dc.titleHeparin and dextran sulfate: their role on rsv infectivity
dc.typeOtro


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