Otro
Immunoreactivity of synthetic peptides derived from proteins of Cryptococcus gattii
Registro en:
Future Microbiology. London: Future Medicine Ltd, v. 9, n. 7, p. 871-878, 2014.
1746-0913
10.2217/FMB.14.49
WOS:000341098600010
Autor
Santos de Serpa Brandao, Rafael Melo
Soares Martins, Liline Maria
Andrade, Helida Monteiro de
Faria, Angelica Rosa
Soares Leal, Maria Jose
Silva, Adalberto Socorro da
Wanke, Bodo
Lazera, Marcia dos Santos
Vainstein, Marilene Henning
Mendes, Rinaldo Poncio
Moris, Daniela Vanessa
Cavalcante, Ricardo de Souza
Hadad do Monte, Semiramis Jamil
Resumen
Aim: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides. Materials & methods: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination. Results: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity. Conclusion: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.