Characterization of peroxidase changes in tolerant and susceptible soybeans challenged by soybean aphid (Hemiptera: Aphididae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMarchi-Werle, L.
dc.creatorHeng-Moss, T. M.
dc.creatorHunt, T. E.
dc.creatorBaldin, Edson Luiz Lopes
dc.creatorBaird, L. M.
dc.date2015-03-18T15:56:09Z
dc.date2016-10-25T20:35:21Z
dc.date2015-03-18T15:56:09Z
dc.date2016-10-25T20:35:21Z
dc.date2014-10-01
dc.date.accessioned2017-04-06T07:17:37Z
dc.date.available2017-04-06T07:17:37Z
dc.identifierJournal Of Economic Entomology. Annapolis: Entomological Soc Amer, v. 107, n. 5, p. 1985-1991, 2014.
dc.identifier0022-0493
dc.identifierhttp://hdl.handle.net/11449/117439
dc.identifierhttp://acervodigital.unesp.br/handle/11449/117439
dc.identifier10.1603/EC14220
dc.identifierWOS:000343097000033
dc.identifierWOS000343097000033.pdf
dc.identifierhttp://dx.doi.org/10.1603/EC14220
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/928086
dc.descriptionChanges in protein content, peroxidase activity, and isozyme profiles in response to soybean aphid feeding were documented at V1 (fully developed leaves at unifoliate node, first trifoliate leaf unrolled) and V3 (fully developed leaf at second trifoliate node, third trifoliate leaf unrolled) stages of soybean aphid-tolerant (KS4202) and -susceptible (SD76R) soybeans. Protein content was similar between infested and control V1 and V3 stage plants for both KS4202 and SD76R at 6, 16, and 22 d after aphid introduction. Enzyme kinetics studies documented that control and aphid-infested KS4202 V1 stage and SD76R V1 and V3 stages had similar levels of peroxidase activity at the three time points evaluated. In contrast, KS4202 aphid-infested plants at the V3 stage had significantly higher peroxidase activity levels than control plants at 6 and 22 d after aphid introduction. The differences in peroxidase activity observed between infested and control V3 stage KS4202 plants at these two time points suggest that peroxidases may be playing multiple roles in the tolerant plant. Native gels stained for peroxidase were able to detect differences in the isozyme profiles of aphid-infested and control plants for both KS4202 and SD76R.
dc.languageeng
dc.publisherEntomological Soc Amer
dc.relationJournal Of Economic Entomology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectperoxidase
dc.subjecthost plant resistance
dc.subjecttolerance
dc.subjectsoybean aphid
dc.titleCharacterization of peroxidase changes in tolerant and susceptible soybeans challenged by soybean aphid (Hemiptera: Aphididae)
dc.typeOtro


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