Antibiofilm activity of irrigating solutions associated with cetrimide. Confocal laser scanning microscopy

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGuerreiro-Tanomaru, J. M.
dc.creatorNascimento, C. A.
dc.creatorFaria-Junior, N. B.
dc.creatorGraeff, M. S. Z.
dc.creatorWatanabe, E.
dc.creatorTanomaru-Filho, M.
dc.date2015-03-18T15:55:13Z
dc.date2016-10-25T20:32:50Z
dc.date2015-03-18T15:55:13Z
dc.date2016-10-25T20:32:50Z
dc.date2014-11-01
dc.date.accessioned2017-04-06T07:13:21Z
dc.date.available2017-04-06T07:13:21Z
dc.identifierInternational Endodontic Journal. Hoboken: Wiley-blackwell, v. 47, n. 11, p. 1058-1063, 2014.
dc.identifier0143-2885
dc.identifierhttp://hdl.handle.net/11449/117113
dc.identifierhttp://acervodigital.unesp.br/handle/11449/117113
dc.identifier10.1111/iej.12248
dc.identifierWOS:000343760600006
dc.identifierhttp://dx.doi.org/10.1111/iej.12248
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/927760
dc.descriptionAimTo evaluate the antibiofilm activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) solutions associated with cetrimide (CTR), and QMiX using confocal laser scanning microscopy.MethodologyEnterococcus faecalis (ATCC- 29212) biofilms were induced on bovine dentine blocks for 14days. The dentine blocks containing biofilm were immersed for 1min in the following solutions: 2.5% NaOCl; 2.5% NaOCl+0.2% CTR; 2% CHX; 2% CHX+0.2% CTR; 0.2% CTR; QMiX. After contact with the solutions, the dentine blocks were stained with Live/Dead((R)) BacLight for analysis of the remaining biofilm using confocal laser scanning microscope. Images were evaluated using the BioImage_L software to determine the total biovolume (m(3)), the green biovolume (live cells) (m(3)) and the percentage of substrate coverage (%). The data were subjected to nonparametric statistical test using Kruskal-Wallis and Dunn's tests at 5% significance level.ResultsAfter exposure to irrigants, the total biovolume observed for CHX, CHX+CTR, CTR, QMiX was similar to distilled water (P>0.05). NaOCl and NaOCl+CTR had the lowest total and green biovolume. The CTR and QMiX had intermediate green biovolume, with greater antibacterial activity than CHX and CHX+CTR (P<0.05). The NaOCl and NaOCl+CTR solutions were associated with microorganism removal and substrate cleaning ability.ConclusionsNaOCl and NaOCl+CTR solutions were effective on microorganism viability and were able to eliminate biofilm. The addition of cetrimide did not influence antibacterial activity.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationInternational Endodontic Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbiofilm
dc.subjectchlorhexidine
dc.subjectconfocal laser scanning microscopy
dc.subjectEnterococcus faecalis
dc.subjectsodium hypochlorite
dc.titleAntibiofilm activity of irrigating solutions associated with cetrimide. Confocal laser scanning microscopy
dc.typeOtro


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