Inflammatory and Cell-Mediated Immune Responses in the Respiratory Tract of Chickens to Infection with Avian Infectious Bronchitis Virus

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOkino, Cintia Hiromi
dc.creatorSantos, Igor Leonardo dos
dc.creatorFernando, Filipe Santos
dc.creatorAlessi, Antonio Carlos
dc.creatorWang, Xiuqing
dc.creatorMontassier, Helio Jose
dc.date2015-03-18T15:54:42Z
dc.date2016-10-25T20:32:00Z
dc.date2015-03-18T15:54:42Z
dc.date2016-10-25T20:32:00Z
dc.date2014-10-01
dc.date.accessioned2017-04-06T07:10:24Z
dc.date.available2017-04-06T07:10:24Z
dc.identifierViral Immunology. New Rochelle: Mary Ann Liebert, Inc, v. 27, n. 8, p. 383-391, 2014.
dc.identifier0882-8245
dc.identifierhttp://hdl.handle.net/11449/116997
dc.identifierhttp://acervodigital.unesp.br/handle/11449/116997
dc.identifier10.1089/vim.2014.0054
dc.identifierWOS:000342507800004
dc.identifierhttp://dx.doi.org/10.1089/vim.2014.0054
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/927644
dc.descriptionTracheal mucosa is the primary site of replication of avian infectious bronchitis virus (IBV), which leads to both morphologic and immune modulatory changes in this organ. To increase the understanding of the mechanisms involved in these processes, we focused on the evaluation of local inflammatory and cell-mediated immune responses after challenge with the M41 strain of IBV, associating these responses with pathologic changes in the tracheal mucosa. At 24 h post-infection, inflammatory cytokines related genes were significantly upregulated, including peaks of TNFSF15 and TGF beta mRNA production, although no tracheal microscopic alterations were observed and only a slightly increase in viral load occurred. At 3 days post-infection (dpi), we observed that the highest upregulation of IL6, IL1 beta, and IFN gamma coincided with highest scores of viral load and microscopic lesions, suggesting a role of both these cytokines and virus load on the development of tracheal lesions. Later, at 7 dpi, the most prominent increases of CD8 alpha alpha mRNA and Granzyme homolog A mRNA were followed by a significant decrease of scores of tracheal lesions and viral load. In conclusion, an early upregulation of expression of proinflammatory cytokines such as IL6, IL1 beta, and IFN gamma induced by the M41 strain of IBV may be partially implicated in the viral pathogenicity on trachea tissues of nonimmune challenged chickens, in addition to a late induction of a putative protective immune responses by this virus through upregulation of CD8 alpha alpha and Granzyme homolog A genes in this organ.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherMary Ann Liebert, Inc
dc.relationViral Immunology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleInflammatory and Cell-Mediated Immune Responses in the Respiratory Tract of Chickens to Infection with Avian Infectious Bronchitis Virus
dc.typeOtro


Este ítem pertenece a la siguiente institución