Is there post-meiotic transcriptional activity during hemipteran spermiogenesis?

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorChaboli Alevi, Kaio Cesar
dc.creatorMendonaca, Priscila Pasqueetto
dc.creatorPereira, Nathalia Paiva
dc.creatorRosa, João Aristeu da
dc.creatorVilela de Azeredo-Oliveira, Maria Tercilia
dc.date2015-03-18T15:54:33Z
dc.date2016-10-25T20:28:27Z
dc.date2015-03-18T15:54:33Z
dc.date2016-10-25T20:28:27Z
dc.date2014-09-01
dc.date.accessioned2017-04-06T07:09:14Z
dc.date.available2017-04-06T07:09:14Z
dc.identifierInvertebrate Reproduction & Development. Abingdon: Taylor & Francis Ltd, v. 58, n. 3, p. 193-198, 2014.
dc.identifier0792-4259
dc.identifierhttp://hdl.handle.net/11449/116947
dc.identifierhttp://acervodigital.unesp.br/handle/11449/116947
dc.identifier10.1080/07924259.2014.889767
dc.identifierWOS:000338105700005
dc.identifierhttp://dx.doi.org/10.1080/07924259.2014.889767
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/927594
dc.descriptionDifferent techniques have been used to evaluate post-meiotic nucleolar activity during spermatogenesis. However, the results have not been consistent, and transcriptional activity during spermiogenesis is still a highly debated issue. Through the use of cytogenetic tools, we evaluated whether the nucleolus is active during spermiogenesis in triatomines, more specifically, in Triatoma lenti. During spermatid differentiation, the nucleolus was observed using the cytogenetic technique of silver staining. Through the Feulgen reaction, we found that the silver ions had labeled the ribosomal DNA (rDNA). Using molecular cytogenetic techniques (DAPI and FISH), we were able to highlight the nucleolus and the Nucleolus Organizing Region (NOR) in spermatid images. Using the C-banding technique, we found rDNA and the NOR to be completely heterochromatic. Since this nuclear structure is present throughout the spermatid stage, we believe that the rDNA and NOR are inactivated by epigenetic factors, because we found a direct relationship between the nucleolus, rDNA, the NOR, and heterochromatin. Because heterochromatization of DNA is an important way to inactivate genetic material, we believe that the entire transcriptional activity required for cellular differentiation during spermiogenesis is mediated by the chromatoid body.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherTaylor & Francis Ltd
dc.relationInvertebrate Reproduction & Development
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcytogenetics
dc.subjectnucleolus
dc.subjectepigenetics
dc.subjectchromatoid body
dc.titleIs there post-meiotic transcriptional activity during hemipteran spermiogenesis?
dc.typeOtro


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