Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBasso, Fernanda Goncalves
dc.creatorSilveira Turrioni, Ana Paula
dc.creatorSoares, Diana Gabiela
dc.creatorBagnato, Vanderlei Salvador
dc.creatorHebling, Josimeri
dc.creatorSouza Costa, Carlos Alberto de
dc.date2015-03-18T15:53:00Z
dc.date2016-10-25T20:24:24Z
dc.date2015-03-18T15:53:00Z
dc.date2016-10-25T20:24:24Z
dc.date2014-10-01
dc.date.accessioned2017-04-06T07:00:57Z
dc.date.available2017-04-06T07:00:57Z
dc.identifierSupportive Care In Cancer. New York: Springer, v. 22, n. 10, p. 2741-2748, 2014.
dc.identifier0941-4355
dc.identifierhttp://hdl.handle.net/11449/116272
dc.identifierhttp://acervodigital.unesp.br/handle/11449/116272
dc.identifier10.1007/s00520-014-2267-3
dc.identifierWOS:000341713400023
dc.identifierhttp://dx.doi.org/10.1007/s00520-014-2267-3
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/926921
dc.descriptionClinical studies have shown that low-level laser therapy (LLLT) can improve local tissue healing of bisphosphonate-induced osteonecrosis of the jaw. However, the effects of laser irradiation on bisphosphonate-treated osteoblasts have not been completely elucidated.Human osteoblasts were cultured in plain culture medium (DMEM). After 48 h, plain DMEM was replaced by DMEM with no fetal bovine serum, for a 24-h incubation followed by addition of zoledronic acid (5 mu M) for additional 48 h. Cells were subjected to LLLT (InGaAsP; 780 +/- 3 nm; 0.025 W) at 0.5, 1.5, 3, 5, and 7 J/cm(2), three times every 24 h. Cell viability, total protein production, alkaline phosphatase activity (ALP), mineral nodule formation, gene expression of collagen type I and ALP, and cell morphology were evaluated.LLLT at 0.5 J/cm(2) increased cell viability of cultured osteoblasts. ALP activity and gene expression, in addition to mineral nodule formation and Col-I gene expression, were not increased by LLLT. LLLT applied to ZA-treated cells increased Col-I expression at 0.5, 1.5, and 3 J/cm(2) but did not improve any other cell activity assessed.LLLT showed limited effects on bisphosphonate-treated osteoblasts.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSpringer
dc.relationSupportive Care In Cancer
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBisphosphonates
dc.subjectOsteonecrosis
dc.subjectOsteoblasts
dc.subjectLow-level laser therapy
dc.subjectCell culture
dc.titleLow-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid
dc.typeOtro


Este ítem pertenece a la siguiente institución