Differential gene expression in Acidithiobacillus ferrooxidans LR planktonic and attached cells in the presence of chalcopyrite

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOssa Henao, Diana Marcela
dc.creatorVicentini, Renato
dc.creatorRodrigues, Viviane Drumond
dc.creatorBevilaqua, Denise
dc.creatorMariscal Ottoboni, Laura Maria
dc.date2015-03-18T15:52:34Z
dc.date2016-10-25T20:23:35Z
dc.date2015-03-18T15:52:34Z
dc.date2016-10-25T20:23:35Z
dc.date2014-07-01
dc.date.accessioned2017-04-06T06:58:33Z
dc.date.available2017-04-06T06:58:33Z
dc.identifierJournal Of Basic Microbiology. Hoboken: Wiley-blackwell, v. 54, n. 7, p. 650-657, 2014.
dc.identifier0233-111X
dc.identifierhttp://hdl.handle.net/11449/116203
dc.identifierhttp://acervodigital.unesp.br/handle/11449/116203
dc.identifier10.1002/jobm.201300871
dc.identifierWOS:000339667600004
dc.identifierhttp://dx.doi.org/10.1002/jobm.201300871
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/926854
dc.descriptionAcidithiobacillus ferrooxidans is commonly used in bioleaching operations to recover copper from sulfide ores. It is commonly accepted that A. ferrooxidans attaches to mineral surfaces by means of extracellular polymeric substances (EPS), however the role of type IV pili and tight adherence genes in this process is poorly understood. Genes related to the formation of type IV pili and tight adherence were identified in the genome of the bacterium, and in this work, we show that A. ferrooxidans actively expresses these genes, as demonstrated by quantitative real-time PCR analysis using cells incubated with chalcopyrite for 2 h. Significant differences in gene expression were observed between planktonic and adhered cells, with the level of expression being much greater in planktonic cells. These results might indicate that planktonic cells can actively adhere to the substrate. A bioinformatics analysis of interaction networks of the tight adherence and type IV pilus assembly genes revealed a strong relationship between conjugation systems (tra operon) and regulatory systems (PilR, PilS).
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationJournal Of Basic Microbiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcidithiobacillus ferrooxidans
dc.subjectType IV pili
dc.subjectChalcopyrite
dc.subjectGene expression
dc.subjectReal-time PCR
dc.titleDifferential gene expression in Acidithiobacillus ferrooxidans LR planktonic and attached cells in the presence of chalcopyrite
dc.typeOtro


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