Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRocha, Ticiana Silva
dc.creatorBaptista, Ana Angelita Sampaio
dc.creatorDonato, Tais Cremasco
dc.creatorMilbradt, Elisane Lenita
dc.creatorOkamoto, Adriano Sakai
dc.creatorAndreatti Filho, Raphael Lucio
dc.date2015-02-02T12:39:27Z
dc.date2016-10-25T20:16:54Z
dc.date2015-02-02T12:39:27Z
dc.date2016-10-25T20:16:54Z
dc.date2014-09-01
dc.date.accessioned2017-04-06T06:42:44Z
dc.date.available2017-04-06T06:42:44Z
dc.identifierBrazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 1065-1073, 2014.
dc.identifier1517-8382
dc.identifierhttp://hdl.handle.net/11449/114349
dc.identifierhttp://acervodigital.unesp.br/handle/11449/114349
dc.identifier10.1590/S1517-83822014000300040
dc.identifierS1517-83822014000300040
dc.identifierS1517-83822014000300040.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822014000300040
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/925062
dc.descriptionIn the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherSociedade Brasileira de Microbiologia
dc.relationBrazilian Journal of Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLactobacillus
dc.subjectidentification
dc.subjectintestinal adhesion
dc.titleIdentification and adhesion profile of Lactobacillus spp. strains isolated from poultry
dc.typeOtro


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