High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDias Siqueira, Vera Lucia
dc.creatorCardoso, Rosilene Fressatti
dc.creatorFalleiros de Padua, Rubia Andreia
dc.creatorCaleffi-Ferracioli, Katiany Rizzieri
dc.creatorHelbel, Cesar
dc.creatorBarreto Santos, Adolfo Carlos
dc.creatorAoki, Elisabeth Eyko
dc.creatorNakamura, Celso Vataru
dc.date2014-12-03T13:11:42Z
dc.date2016-10-25T20:14:54Z
dc.date2014-12-03T13:11:42Z
dc.date2016-10-25T20:14:54Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T06:33:32Z
dc.date.available2017-04-06T06:33:32Z
dc.identifierBrazilian Journal of Pharmaceutical Sciences. São Paulo: Univ São Paulo, Conjunto Quimicas, v. 49, n. 1, p. 49-56, 2013.
dc.identifier1984-8250
dc.identifierhttp://hdl.handle.net/11449/113443
dc.identifierhttp://acervodigital.unesp.br/handle/11449/113443
dc.identifierWOS:000324586200006
dc.identifierS1984-82502013000100006.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1984-82502013000100006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/924185
dc.descriptionIn Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Parana, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-beta-lactamase (MBL) was detected using a disk approximation test. Genotyping was performed using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Approximately 55% of the P. aeruginosa isolates and 92% of the Acinetobacter spp. isolates were multiresistant, but none were MBL-producers. ERIC-PCR revealed the presence of small clusters of carbapenem-resistant Acinetobacter spp., most likely OXA-type carbapenemase producers. Furthermore, high genetic diversity in P. aeruginosa and Acinetobacter spp. clinical isolates was observed, suggesting that cross-transmission is not very frequent in the studied hospital.
dc.languageeng
dc.publisherUniversidade de São Paulo (USP), Conjunto Quimicas
dc.relationBrazilian Journal of Pharmaceutical Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPseudomonas aeruginosa/antimicrobial resistance profile
dc.subjectPseudomonas aeruginosa/genetic study
dc.subjectAcinetobacter spp./antimicrobial resistance profile
dc.subjectAcinetobacter spp./genetic study
dc.subjectAntimicrobial resistance
dc.subjectBacterial typing
dc.titleHigh genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil
dc.typeOtro


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