Safety assessment of oral photodynamic therapy in rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFontana, Carla R.
dc.creatorLerman, Mark A.
dc.creatorPatel, Niraj
dc.creatorGrecco, Clovis
dc.creatorSouza Costa, Carlos A. de
dc.creatorAmiji, Mansoor M.
dc.creatorBagnato, Vanderlei S.
dc.creatorSoukos, Nikolaos S.
dc.date2014-12-03T13:11:42Z
dc.date2016-10-25T20:14:52Z
dc.date2014-12-03T13:11:42Z
dc.date2016-10-25T20:14:52Z
dc.date2013-02-01
dc.date.accessioned2017-04-06T06:33:28Z
dc.date.available2017-04-06T06:33:28Z
dc.identifierLasers In Medical Science. London: Springer London Ltd, v. 28, n. 2, p. 479-486, 2013.
dc.identifier0268-8921
dc.identifierhttp://hdl.handle.net/11449/113435
dc.identifierhttp://acervodigital.unesp.br/handle/11449/113435
dc.identifier10.1007/s10103-012-1091-6
dc.identifierWOS:000315579400018
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1091-6
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/924177
dc.descriptionPhotodynamic therapy (PDT) is based on the synergism of a photosensitive drug (a photosensitizer) and visible light to destroy target cells (e.g., malignant, premalignant, or bacterial cells). The aim of this study was to investigate the response of normal rat tongue mucosa to PDT following the topical application of hematoporphyrin derivative (PhotogemA (R)), PhotodithazineA (R), methylene blue (MB), and poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with MB. One hundred and thirty three rats were randomly divided in various groups: the PDT groups were treated with the photosensitizers for 10 min followed by exposure to red light. Those in control groups received neither photosensitizer nor light, and they were subjected to light exposure alone or to photosensitizer alone. Fluorescent signals were obtained from tongue tissue immediately after the topical application of photosensitizers and 24 h following PDT. Histological changes were evaluated at baseline and at 1, 3, 7, and 15 days post-PDT treatment. Fluorescence was detected immediately after the application of the photosensitizers, but not 24 h following PDT. Histology revealed intact mucosa in all experimental groups at all evaluation time points. The results suggest that there is a therapeutic window where PDT with PhotogemA (R), PhotodithazineA (R), MB, and MB-loaded PLGA nanoparticles could safely target oral pathogenic bacteria without damaging normal oral tissue.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherSpringer
dc.relationLasers in Medical Science
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPhotodynamic therapy
dc.subjectPhotosensitizers
dc.subjectHistological analysis
dc.subjectFluorescence
dc.titleSafety assessment of oral photodynamic therapy in rats
dc.typeOtro


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