Comparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira, Bruno H.
dc.creatorCoradi, Gilberto V.
dc.creatorAttili-Angelis, Derlene
dc.creatorScauri, Carolina
dc.creatorLuques, Ana Helena P. G.
dc.creatorBarbosa, Aneli M.
dc.creatorDekker, Robert F. H.
dc.creatorOliva Neto, Pedro de
dc.creatorLima, Valeria M. G.
dc.date2014-12-03T13:11:37Z
dc.date2016-10-25T20:14:39Z
dc.date2014-12-03T13:11:37Z
dc.date2016-10-25T20:14:39Z
dc.date2013-12-01
dc.date.accessioned2017-04-06T06:32:26Z
dc.date.available2017-04-06T06:32:26Z
dc.identifierEuropean Journal Of Lipid Science And Technology. Hoboken: Wiley-blackwell, v. 115, n. 12, p. 1413-1425, 2013.
dc.identifier1438-7697
dc.identifierhttp://hdl.handle.net/11449/113337
dc.identifierhttp://acervodigital.unesp.br/handle/11449/113337
dc.identifier10.1002/ejlt.201300087
dc.identifierWOS:000328733600008
dc.identifierhttp://dx.doi.org/10.1002/ejlt.201300087
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/924084
dc.descriptionThis study provides a comparison of fermentation conditions for the production lipases by Fusarium sp. (Gibberella fujikuroi complex) isolate FCLA-MA-41 in submerged (SmF) and solid-state fermentation (SSF) using agro-industrial residues. To assess SmF, a univariate study of seven carbon sources (crambe, corn, linseed, olive, palm (dende), soybean oils and chicken fat) and four nitrogen sources (ammonium sulfate, sodium nitrate, urea, yeast extract) was performed. The relationship among the concentrations of carbon and nitrogen sources, Triton X-100 and yeast extract was studied using a Central Composite Design (CCD). To assess SSF, different residues (sugarcane bagasse) and oil-seed meals (castorbean, corn, crambe, soybean) with additional supplements added (minimum salts, seed oils) were examined. Based on the CCD matrix, a medium containing crambe oil (17.5mL/L), Triton X-100 (5g/L), ammonium sulfate (5g/L) and yeast extract (1g/L) was proposed, resulting in a lipase titer of 3.0 +/- 0.25U/mL. The cost/production relationship was 7.73% less expensive than SmF with corn oil. SSF produced a maximum lipase titer of 5.0 +/- 0.25U/gds on crambe meal moistened with phosphate buffer. The best cost/enzyme activity estimate was for SSF with crambe meal as substrate in only distilled water (87.27% less expensive than SmF).
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationEuropean Journal of Lipid Science and Technology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCentral composite design
dc.subjectCorn oil
dc.subjectChicken fat
dc.subjectAmmonium sulfate
dc.subjectTriton X-100
dc.titleComparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)
dc.typeOtro


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