Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTeixeira Rossi, Ana Flavia
dc.creatorDuarte, Marcia Cristina
dc.creatorPoltronieri, Ayla Blanco
dc.creatorValsechi, Marina Curado
dc.creatorJorge, Yvana Cristina
dc.creatorNeto, Dalisio de-Santi
dc.creatorRahal, Paula
dc.creatorOliani, Sonia Maria
dc.creatorSilva, Ana Elizabete
dc.date2014-12-03T13:11:06Z
dc.date2016-10-25T20:12:08Z
dc.date2014-12-03T13:11:06Z
dc.date2016-10-25T20:12:08Z
dc.date2014-01-01
dc.date.accessioned2017-04-06T06:26:27Z
dc.date.available2017-04-06T06:26:27Z
dc.identifierMediators Of Inflammation. New York: Hindawi Publishing Corporation, 11 p., 2014.
dc.identifier0962-9351
dc.identifierhttp://hdl.handle.net/11449/112844
dc.identifierhttp://acervodigital.unesp.br/handle/11449/112844
dc.identifier10.1155/2014/478138
dc.identifierWOS:000332539600001
dc.identifierWOS000332539600001.pdf
dc.identifierhttp://dx.doi.org/10.1155/2014/478138
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/923598
dc.descriptionObjective. Annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU). Methods. Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of ANXA1 and LGALS1 mRNA and protein expression, respectively. Results. Increased relative expression levels of ANXA1 were found in 100% of cases, both in IM (mean RQ = 6.22 +/- 0.06) and in GU (mean RQ = 6.69 +/- 0.10). However, the LGALS1 presented basal expression in both groups (IM: mean RQ = 0.35 +/- 0.07; GU: mean RQ = 0.69 +/- 0.09). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (P < 0.05) but absence or low immunorectivity in normal mucosa. Conclusion. Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.relationMediators of Inflammation
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleDeregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer
dc.typeOtro


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