Xylanase and beta-Xylosidase from Penicillium janczewskii: Production, Physico-chemical Properties, and Application of the Crude Extract to Pulp Biobleaching

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTerrasan, Cesar R. F.
dc.creatorTemer, Beatriz
dc.creatorSarto, Camila
dc.creatorSilva Junior, Francides G.
dc.creatorCarmona, Eleonora C.
dc.date2014-12-03T13:11:02Z
dc.date2016-10-25T20:11:55Z
dc.date2014-12-03T13:11:02Z
dc.date2016-10-25T20:11:55Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T06:25:34Z
dc.date.available2017-04-06T06:25:34Z
dc.identifierBioresources. Raleigh: North Carolina State Univ Dept Wood & Paper Sci, v. 8, n. 1, p. 1292-1305, 2013.
dc.identifier1930-2126
dc.identifierhttp://hdl.handle.net/11449/112756
dc.identifierhttp://acervodigital.unesp.br/handle/11449/112756
dc.identifierWOS:000314632300103
dc.identifierWOS000314632300103.pdf
dc.identifierhttp://www.ncsu.edu/bioresources/BioRes_08/BioRes_08_1_1292_Terrasan_TSSC_Xylanase_Extract_Biobleaching_3172.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/923511
dc.descriptionExtracellular xylanase and beta-xylosidase production by a Penicillium janczewskii strain were investigated in liquid cultures with xylan from oat spelts under different physical and chemical conditions. The selected conditions for optimized production of xylanase and beta-xylosidase were 7 days, pH 6.5, at 30 degrees C and 8 days, pH 5.0, at 25 degrees C, respectively. The xylanase exhibited optimal activity in pH 5.0 at 50 degrees C and the beta-xylosidase in pH 4.0 at 75 degrees C. The xylanase was more stable at pH 6.0 to 9.5, while the beta-xylosidase remained stable at pH ranging from 1.6 to 5.5. The xylanase half-life (T-50) at 40, 50, and 60 degrees C was 183, 15, and 3 min, respectively. beta-xylosidase half-life was 144, 8, and 4 min at 50, 65, and 75 degrees C, respectively. When applied to the biobleaching of Eucalyptus kraft pulp, xylanase dosages of 2 and 4 U/g dried pulp reduced, respectively, kappa number by 3.0 and 3.3 units after 1 h treatment, demonstrating that the use of P. janczewskii xylanases in this process is quite promising. The pulp viscosity was not altered, confirming the absence of cellulolytic enzymes in the fungal extract.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherNorth Carolina State Univ Dept Wood & Paper Sci
dc.relationBioResources
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectXylanolytic enzymes
dc.subjectPenicillium janczewskii
dc.subjectEnzyme production
dc.subjectEnzyme characterization
dc.subjectPulp biobleaching
dc.titleXylanase and beta-Xylosidase from Penicillium janczewskii: Production, Physico-chemical Properties, and Application of the Crude Extract to Pulp Biobleaching
dc.typeOtro


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