Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSakane, K. K.
dc.creatorMonteiro, C. J.
dc.creatorSilva, W.
dc.creatorSilva, A. R.
dc.creatorSantos, P. M.
dc.creatorLima, K. F.
dc.creatorMoraes, K. C. M.
dc.date2014-12-03T13:10:59Z
dc.date2016-10-25T20:11:49Z
dc.date2014-12-03T13:10:59Z
dc.date2016-10-25T20:11:49Z
dc.date2014-01-01
dc.date.accessioned2017-04-06T06:25:05Z
dc.date.available2017-04-06T06:25:05Z
dc.identifierBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 1, p. 50-59, 2014.
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/11449/112703
dc.identifierhttp://acervodigital.unesp.br/handle/11449/112703
dc.identifier10.1590/1414-431X20133028
dc.identifierS0100-879X2013005003028
dc.identifierWOS:000331907500007
dc.identifierS0100-879X2013005003028.pdf
dc.identifierhttp://dx.doi.org/10.1590/1414-431X20133028
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/923463
dc.descriptionCardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 mu M celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relationBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCellular and molecular analyses
dc.subjectCell death
dc.subjectCyclooxygenase-2 inhibitor
dc.subjectH9c2 cardiac cell line
dc.titleCellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms
dc.typeOtro


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