Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira, Jonatas Rafael de
dc.creatorAguiar Almeida, Rosilene Batista de
dc.creatorFigueiredo Vilela, Polyana das Gracas
dc.creatorOliveira, Felipe Eduardo de
dc.creatorRocha, Rosilene Fernandes da
dc.creatorCardoso Jorge, Antonio Olavo
dc.creatorOliveira, Luciane Dias de
dc.date2014-12-03T13:10:55Z
dc.date2016-10-25T20:11:41Z
dc.date2014-12-03T13:10:55Z
dc.date2016-10-25T20:11:41Z
dc.date2014-08-01
dc.date.accessioned2017-04-06T06:24:33Z
dc.date.available2017-04-06T06:24:33Z
dc.identifierArchives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 8, p. 808-814, 2014.
dc.identifier0003-9969
dc.identifierhttp://hdl.handle.net/11449/112648
dc.identifierhttp://acervodigital.unesp.br/handle/11449/112648
dc.identifier10.1016/j.archoralbio.2014.05.013
dc.identifierWOS:000338822300006
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2014.05.013
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/923408
dc.descriptionObjectives: To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).Design: By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1 beta and TNF-alpha by ELISA.Results: The most effective concentration was 250 mg/mL and also promoted significant reduction (log(10)) in the biofilms of S. aureus (0.438 +/- 0.269), S. epiderrnidis (0.377 +/- 0.298), S. mutans (0.244 +/- 0.161) and C. albicans (0.746 +/- 0.209). Cell viability was similar to 100%. The production of IL-beta was similar to the control group (p > 0.05) and there was inhibition of TNF-alpha (p < 0.01).Conclusions: A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages. (C) 2014 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationArchives of Oral Biology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectArctium lappa
dc.subjectBiofilms
dc.subjectStaphylococcus
dc.subjectStreptococcus mutans
dc.subjectCandida
dc.subjectRAW 264.7
dc.titleControl of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)
dc.typeOtro


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