Otro
Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells
Registro en:
Biomed Research International. New York: Hindawi Publishing Corporation, 5 p., 2014.
2314-6133
10.1155/2014/895049
WOS:000330715200001
WOS000330715200001.pdf
Autor
Vargas, Fernanda da Silveira
Soares, Diana Gabriela
Dias Ribeiro, Ana Paula
Hebling, Josimeri
De Souza Costa, Carlos Alberto
Resumen
The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (alpha-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mM alpha-T for 60 minutes. They were then exposed or not to H2O2 for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H2O2 (0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests; alpha = 5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated with alpha-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2 had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect of alpha-T against the H2O2 toxicity. Overall, the vitamin E alpha-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2. The most effective cell protection was provided by 5 and 10 mM concentrations of alpha-T. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)