Isolation, Culture and Differentiation of Buffaloes Bone Marrow Mesenchymal Stem Cells Obtained from the Coxal Tuberosity

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOba, Eunice
dc.creatorListoni, Amanda Jeronimo
dc.creatorMaia, Leandro
dc.creatorMoraes, Carolina Nogueira de
dc.creatorLandim-Alvarenga, Fernanda da Cruz
dc.date2014-12-03T13:10:40Z
dc.date2016-10-25T20:11:04Z
dc.date2014-12-03T13:10:40Z
dc.date2016-10-25T20:11:04Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T06:22:00Z
dc.date.available2017-04-06T06:22:00Z
dc.identifierBuffalo Bulletin. Bangkok: Int Buffalo Information Ctr, v. 32, p. 1128-1130, 2013.
dc.identifier0125-6726
dc.identifierhttp://hdl.handle.net/11449/112388
dc.identifierhttp://acervodigital.unesp.br/handle/11449/112388
dc.identifierWOS:000331980300201
dc.identifierhttp://ibic.lib.ku.ac.th/e-Bulletin/archive.htm
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/923150
dc.descriptionCurrently, much attention has been devoted to the renewal of knowledge about Stem Cells and Cell Therapy in domestic species. In this sense, the present work aimed to develop a methodology for collecting, processing and cultivation of mesenchymal stem cells obtained from bone marrow of coxal tuberosity in buffaloes. The collection was performed using a Komiyashiki needle, which was introduced in the coxal tuberosity and the bone marrow aspirated into a heparinized syringe with the aid of negative pressure. Directly after collection samples were processed at the laboratory at FMVZ - UNESP. The samples took approximately 32 days to reach 80% confluence, when the first passage and differentiation was performed. To confirm the mesenchymal origin, cells were induced to differentiate into adipogenic and osteogenic lineages. Samples showed morphological changes during differentiation protocol, but not all presented production of extracellular deposits of calcium or intracellular fat droplets, observed after staining with Alizarin Red and Oil Red respectively. Compared with the material obtained from other species and processed in the same laboratory, the primary culture was longer. Therefore, more studies are needed to standardize the age of animals used and to test other inducers of cell differentiation.
dc.languageeng
dc.publisherInt Buffalo Information Ctr
dc.relationBuffalo Bulletin
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbone marrow
dc.subjectbuffalo
dc.subjectcoxal tuberosity
dc.subjectcell differentiation stem cells
dc.titleIsolation, Culture and Differentiation of Buffaloes Bone Marrow Mesenchymal Stem Cells Obtained from the Coxal Tuberosity
dc.typeOtro


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