Transforming Growth Factor Beta (TGFβ1) and Epidermal Growth Factor (EGF) as Biomarkers of Leishmania (V) braziliensis Infection and Early Therapeutic Response in Cutaneous Leishmaniasis: Studies in Hamsters

dc.creatorRobledo Restrepo, Sara María
dc.creatorMontoya Restrepo, Edwin Andrés
dc.creatorYepes Daza, Lina Marcela
dc.creatorBedoya, Alexander
dc.creatorHenao, Raúl
dc.creatorDelgado Murcia, Lucy Gabriela
dc.creatorVélez Bernal, Iván Darío
dc.date2023-05-16T16:21:26Z
dc.date2023-05-16T16:21:26Z
dc.date2018
dc.date.accessioned2024-04-23T18:21:03Z
dc.date.available2024-04-23T18:21:03Z
dc.identifierMontoya A, Yepes L, Bedoya A, Henao R, Delgado G, Vélez ID and Robledo SM (2018) Transforming Growth Factor Beta (TGFβ1) and Epidermal Growth Factor (EGF) as Biomarkers of Leishmania (V) braziliensis Infection and Early Therapeutic Response in Cutaneous Leishmaniasis: Studies in Hamsters. Front. Cell. Infect. Microbiol. 8:350. doi: 10.3389/fcimb.2018.00350
dc.identifier2235-2988
dc.identifierhttps://hdl.handle.net/10495/35029
dc.identifier10.3389/fcimb.2018.00350
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9230734
dc.descriptionABSTRACT: Introduction: In cutaneous leishmaniasis, the host immune response is responsible for the development of skin injuries but also for resolution of the disease especially after antileishmanial therapy. The immune factors that participate in the regulation of inflammation, remodeling of the extracellular matrix, cell proliferation and differentiation may constitute biomarkers of diseases or response to treatment. In this work, we analyzed the production of the growth factors EGF, TGFβ1, PDGF, and FGF during the infection by Leishmania parasites, the development of the injuries and the early response to treatment. Methodology: Golden hamsters were infected with L. (V) braziliensis. The growth factors were detected in skin scrapings and biopsies every 2 weeks after infected and then at day 7 of treatment with different drug candidates by RT-qPCR. The parasitic load was also quantified by RT-qPCR in skin biopsies sampled at the end of the study. Results: The infection by L. (V) braziliensis induced the expression of all the growth factors at day 15 of infection. One month after infection, EGF and TGFβ1 were expressed in all hamsters with inverse ratio. While the EGF and FGF levels decreased between day 15 and 30 of infection, the TGFβ1 increased and the PGDF levels did not change. The relative expression of EGF and TGFβ1 increased notably after treatment. However, the increase of EGF was associated with clinical cure while the increase of TGFβ1 was associated with failure to treatment. The amount of parasites in the cutaneous lesion at the end of the study decreased according to the clinical outcome, being lower in the group of cured hamsters and higher in the group of hamsters that had a failure to the treatment. Conclusions: A differential profile of growth factor expression occurred during the infection and response to treatment. Higher induction of TGFβ1 was associated with active disease while the higher levels of EGF are associated with adequate response to treatment. The inversely EGF/TGFβ1 ratio may be an effective biomarker to identify establishment of Leishmania infection and early therapeutic response, respectively. However, further studies are needed to validate the utility of the proposed biomarkers in field conditions.
dc.descriptionCOL0015099
dc.format10
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.publisherPrograma de Estudio y Control de Enfermedades Tropicales (PECET)
dc.publisherLausana, Suiza
dc.relationFront. Cell. Infect. Microbiol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectIntercellular Signaling Peptides and Proteins
dc.subjectPéptidos y Proteínas de Señalización Intercelular
dc.subjectLeishmaniasis, Cutaneous
dc.subjectLeishmaniasis Cutánea
dc.subjectBiomarkers
dc.subjectBiomarcadores
dc.subjectLeishmania braziliensis
dc.subjectEpidermal Growth Factor
dc.subjectFactor de Crecimiento Epidérmico
dc.subjectReceptors, Fibroblast Growth Factor
dc.subjectReceptores de Factores de Crecimiento de Fibroblastos
dc.subjectTransforming Growth Factor beta
dc.subjectFactor de Crecimiento Transformador beta
dc.subjectReceptors, Platelet-Derived Growth Factor
dc.subjectReceptores del Factor de Crecimiento Derivado de Plaquetas
dc.titleTransforming Growth Factor Beta (TGFβ1) and Epidermal Growth Factor (EGF) as Biomarkers of Leishmania (V) braziliensis Infection and Early Therapeutic Response in Cutaneous Leishmaniasis: Studies in Hamsters
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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