Differential Replication of Dengue Virus Serotypes 2 and 3 in Coinfections of C6/36 Cells and Aedes Aegypti Mosquitoes

dc.creatorQuintero Gil, Diana Carolina
dc.creatorMartínez Gutiérrez, Marlen
dc.creatorOsorio Benítez, Jorge Emilio
dc.creatorOspina Ospina, Marta Cecilia
dc.date2023-03-29T19:55:19Z
dc.date2023-03-29T19:55:19Z
dc.date2014
dc.date.accessioned2024-04-23T18:16:38Z
dc.date.available2024-04-23T18:16:38Z
dc.identifierQuintero-Gil DC, Ospina M, Osorio-Benitez JE, Martinez-Gutierrez M. Differential replication of dengue virus serotypes 2 and 3 in coinfections of C6/36 cells and Aedes aegypti mosquitoes. J Infect Dev Ctries. 2014 Jul 14;8(7):876-84. doi: 10.3855/jidc.3978.
dc.identifier2036-6590
dc.identifierhttps://hdl.handle.net/10495/34349
dc.identifier10.3855/jidc.3978
dc.identifier1972-2680
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9230603
dc.descriptionABSTRACT: Introduction: Different dengue virus (DENV) serotypes have been associated with greater epidemic potential. In turn, the increased frequency in cases of severe forms of dengue has been associated with the cocirculation of several serotypes. Because Colombia is a country with an endemic presence of all four DENV serotypes, the aim of this study was to evaluate the in vivo and in vitro replication of the DENV-2 and DENV-3 strains under individual infection and coinfection conditions. Methodology: C6/36HT cells were infected with the two strains individually or simultaneously (coinfection). Replication capacity was evaluated by RT-qPCR, and the effects on cell viability were assessed with an MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Additionally, Aedes aegypti mosquitoes were artificially fed the two strains of each serotype individually or simultaneously. The viral genomes were quantified by RT-qPCR and the survival of the infected mosquitoes was compared to that of uninfected controls. Results: In single infections, three strains significantly affected C6/36HT cell viability, but no significant differences were found in the replication capacities of the strains of the same serotype. In the in vivo infections, mosquito survival was not affected, and no significant differences in replication between strains of the same serotype were found. Finally, in coinfections, serotype 2 replicated with a thousandfold greater efficiency than serotype 3 did both in vitro and in vivo. Conclusions: Due to the cocirculation of serotypes in endemic regions, further studies of coinfections in a natural environment would further an understanding of the transmission dynamics that affect DENV infection epidemiology.
dc.descriptionCOL0015099
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherOpen Learning on Enteric Pathogens
dc.publisherPrograma de Estudio y Control de Enfermedades Tropicales (PECET)
dc.publisherItalia
dc.relationJ. Infect. Dev. Ctries.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectDengue Virus
dc.subjectVirus del Dengue
dc.subjectAedes
dc.subjectVirus Replication
dc.subjectReplicación Viral
dc.titleDifferential Replication of Dengue Virus Serotypes 2 and 3 in Coinfections of C6/36 Cells and Aedes Aegypti Mosquitoes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


Este ítem pertenece a la siguiente institución