dc.creatorTorres Gómez, Isaura Paticia
dc.creatorGarcía Cepero, Ana María
dc.creatorHernández Ruiz, Orville
dc.creatorGonzález Marín, Angel Augusto
dc.creatorMcEwen Ochoa, Juan Guillermo
dc.creatorRestrepo Moreno, Ángela
dc.creatorArango Arteaga, Myrtha
dc.date2023-03-06T19:55:01Z
dc.date2023-03-06T19:55:01Z
dc.date2010
dc.date.accessioned2024-04-23T17:56:11Z
dc.date.available2024-04-23T17:56:11Z
dc.identifier1087-1845
dc.identifierhttps://hdl.handle.net/10495/33724
dc.identifier10.1016/j.fgb.2009.11.005
dc.identifier1096-0937
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S108718450900190X#!
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9230108
dc.descriptionABSTRACT Paracoccidioides brasiliensis has been classified in the phylum Ascomycota, order Onygenales, family Ajellomycetaceae, even in the absence of a known sexual cycle or mating system. The objective of this work was to determine the presence of the mating type locus in 71 P. brasiliensis isolates from various sources. A PCR assay using specific primers for the MAT 1 gene was developed and applied for the detection of such genes. Two heterothallic groups (MAT1-1 or MAT1-2) were recognized and, in some isolates, gene expression was confirmed indicating the existence of a basal gene expression. The distribution of two mating type loci in the studied population suggested that sexual reproduction might occur in P. brasiliensis. This finding points towards the possibility of applying a more precise definition of the concept of biological species to P. brasiliensis. Further studies should be conducted to confirm the sexual capacity of this fungus and its implications among phylogenetic species and geographical distribution.
dc.descriptionCOL0000962
dc.format8
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.publisherBiología Celular y Molecular CIB U. de A. U. del Rosario
dc.publisherOrlando, Estados Unidos
dc.relationFungal Genet. Biol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParacoccidioides
dc.subjectGene Expression
dc.subjectExpresión Génica
dc.titlePresence and Expression of the Mating Type Locus in Paracoccidioides Brasiliensis Isolates
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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