Plant cell suspension culture of Thevetia peruviana (Pers.) K. Schum. in shake flask and stirred tank reactor scale: A comparative study

dc.creatorArias Echeverri, Juan Pablo
dc.creatorZapata Acosta, Karol
dc.creatorRojano, Benjamín Alberto
dc.creatorPeñuela Vásquez, Mariana
dc.creatorArias Zabala, Mario
dc.date2023-02-28T19:47:12Z
dc.date2023-02-28T19:47:12Z
dc.date2017
dc.date.accessioned2024-04-23T17:55:46Z
dc.date.available2024-04-23T17:55:46Z
dc.identifierArias, J. P., Zapata, K., Rojano, B., Peñuela, M., & Arias, M. (2017). Plant cell suspension culture of Thevetia peruviana (Pers.) K. Schum. in shake flask and stirred tank reactor scale: A comparative study. African Journal of Biotechnology , 16(52), 2355-2363.
dc.identifierhttps://hdl.handle.net/10495/33630
dc.identifier10.5897/AJB2017.16281
dc.identifier1684-5315
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9230092
dc.descriptionABSTRACT: The aim of this work was to evaluate the effect of changing the shake flask for a stirred-tank bench bioreactor on the performance of Thevetia peruviana cultures. Maximum cell growth of 18.59 and 8.46 g DW/L for shake flask and bench bioreactor at day 14 was obtained, respectively. The end of the exponential growth phase in shake flask was correlated with total sugar consumption, while this behavior was not observed in bench bioreactor. Preferential uptake of glucose over fructose was observed in both systems. Cell morphology was similar for both systems during culture time, exhibiting individual cells with cylindrical and elongated shape at day 0 and some aggregates with rounded cells at day 14. Polyphenol production was not affected by the system configuration and scale. Intra and extracellular antioxidant activity were directly related with phenol production in both systems. Guaiacol peroxidase activity (GPX) went from 1.87 to 7.97 µM/min/mg protein in shake flask and it correlated with low reactive oxygen species (ROS) production. This is an indication of a positive enzymatic cell response for culture in shake flask mediated by GPX. In contrast, the conditions in the bench bioreactor generated a higher stress environment, increasing ROS production without activating enzymatic or non-enzymatic responses led by guaiacol and phenolic compounds, respectively. In conclusion, bench stirred-tank bioreactor scale affects biomass production and sugars uptake negatively.
dc.descriptionCOL0023715
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherAcademic Journals
dc.publisherBioprocesos
dc.publisherNigeria
dc.relationAfr. J. Biotechnol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectReactores Biológicos
dc.subjectBioreactors
dc.subjectCompuestos fenólicos
dc.subjectPhenolic Compounds
dc.subjectCélulas Vegetales
dc.subjectPlant Cells
dc.subjectCultivo en suspensión
dc.subjectSuspension culture
dc.subjectThevetia peruviana
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_34130
dc.titlePlant cell suspension culture of Thevetia peruviana (Pers.) K. Schum. in shake flask and stirred tank reactor scale: A comparative study
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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