Molecular characterization of Marek’s disease virus in a poultry layer farm from Colombia

dc.creatorLópez Osorio, Sara
dc.creatorPiedrahita, Diego
dc.creatorEspinal Restrepo, María Adelaida
dc.creatorRamírez Nieto, Gloria Consuelo
dc.creatorAranzazu Taborda, Diego Alfonso
dc.creatorChaparro Gutiérrez, Jenny Jovana
dc.creatorNair, Venugopal
dc.creatorWilliams, Susan M.
dc.creatorBaigent, Susan
dc.creatorVentura Polite, César
dc.date2023-03-07T20:28:52Z
dc.date2023-03-07T20:28:52Z
dc.date2017
dc.date.accessioned2024-04-23T17:53:57Z
dc.date.available2024-04-23T17:53:57Z
dc.identifierLópez-Osorio S, Piedrahita D, Espinal-Restrepo MA, Ramírez-Nieto GC, Nair V, Williams SM, Baigent S, Ventura-Polite C, Aranzazu-Taborda DA, Chaparro-Gutiérrez JJ. Molecular characterization of Marek's disease virus in a poultry layer farm from Colombia. Poult Sci. 2017 Jun 1;96(6):1598-1608. doi: 10.3382/ps/pew464.
dc.identifier0032-5791
dc.identifierhttps://hdl.handle.net/10495/33768
dc.identifier10.3382/ps/pew464
dc.identifier1525-3171
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9230040
dc.descriptionABSTRACT. Marek’s disease (MD) is a lymphopro- liferative disease caused by an Alphaherpesvirus, genus Mardivirus, serotype 1 (Gallid Herpesvirus 2, GaHV-2) that includes all known pathogenic strains. In addition to Marek’s disease virus (MDV) serotype 1, the genus includes 2 distinct nonpathogenic serotypes: serotype 2 (GaHV-3) and serotype 3 (Meleagridis Herpesvirus 1, MeHV-1) which are used in commercially available vaccines against MD. As a result of vaccination, clinical signs are not commonly observed, and new cases are usually associated with emerging variant strains against which the vaccines are less effective. In this study, a commercial layer farm showing clinical signs compatible with MDV infection was evaluated. Histological lesions and positive immunohistochemistry in the sciatic nerve and thymus were compatible with cytolytic phase of MD. GaHV-2, GaHV-3 and MeHV-1 were identified by PCR and qPCR in blood samples from 17 birds with suspected MD. Analysis of the Meq gene of the Colombian GaHV-2 isolate revealed a 99% sequence identity with Asian strains, and in the phylogenetic analysis clustered with vv+ MDV. The analysis of amino acid alignments demonstrated an interruption of the proline rich region in P176A, P217A and P233L positions, which are generally associated with vv+ strains. Some of these changes, such as P233L and L258S positions have not been reported previously. In addition, primary cell cultures inoculated with lymphocytes isolated from the spleen showed typical cytopathic effect of GaHV-2 at 5 d post infection. Based on the molecular analysis, the results from this study indicate the presence of vv+ MDV infection in commercial birds for the first time in Colombia. It is recommended to perform further assays in order to demonstrate the pathotype characteristics in vivo.
dc.descriptionCOL0001262
dc.descriptionCOL0153246
dc.format11
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPoultry Science Association
dc.publisherCENTAURO
dc.publisherCentro de Investigaciones Básicas y Aplicadas en Veterinaria (CIBAV)
dc.publisherChampaign, Estados Unidos
dc.relationPoult. Sci.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCélulas Cultivadas
dc.subjectCells, Cultured
dc.subjectColombia
dc.subjectEmbrión de Pollo
dc.subjectChick Embryo
dc.subjectPollos
dc.subjectChickens - virology
dc.subjectADN Viral
dc.subjectDNA, Viral
dc.subjectHerpesvirus Gallináceo 2
dc.subjectHerpesvirus 2, Gallid
dc.subjectEnfermedad de Marek
dc.subjectMarek Disease
dc.subjectEnfermedades de las Aves de Corral
dc.subjectPoultry Diseases
dc.subjectSciatic Neuropathy
dc.subjectNeuropatía Ciática
dc.subjectAnálisis de Secuencia de ADN
dc.subjectSequence Analysis, DNA
dc.subjectFilogenia
dc.subjectPhylogeny
dc.subjecthttps://id.nlm.nih.gov/mesh/D002478
dc.subjecthttps://id.nlm.nih.gov/mesh/D003105
dc.subjecthttps://id.nlm.nih.gov/mesh/D002642
dc.subjecthttps://id.nlm.nih.gov/mesh/D002645
dc.subjecthttps://id.nlm.nih.gov/mesh/D004279
dc.subjecthttps://id.nlm.nih.gov/mesh/D008381
dc.subjecthttps://id.nlm.nih.gov/mesh/D008380
dc.subjecthttps://id.nlm.nih.gov/mesh/D011201
dc.subjecthttps://id.nlm.nih.gov/mesh/D020426
dc.subjecthttps://id.nlm.nih.gov/mesh/D017422
dc.subjecthttps://id.nlm.nih.gov/mesh/D010802
dc.titleMolecular characterization of Marek’s disease virus in a poultry layer farm from Colombia
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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