Metagenomics Shows That Low-Energy Anaerobic−Aerobic Treatment Reactors Reduce Antibiotic Resistance Gene Levels from Domestic Wastewater

dc.creatorRodríguez Loaiza, Diana Catalina
dc.creatorChristgen, Beate
dc.creatorYang, Ying
dc.creatorAhammad, S. Z.
dc.creatorLi, Bing
dc.creatorZhang, Tong
dc.creatorGraham, David W.
dc.date2023-02-28T17:38:43Z
dc.date2023-02-28T17:38:43Z
dc.date2015
dc.date.accessioned2024-04-23T17:51:40Z
dc.date.available2024-04-23T17:51:40Z
dc.identifierBeate Christgen, Ying Yang, S. Z. Ahammad, Bing Li, D. Catalina Rodriquez, Tong Zhang, and David W. Graham Environmental Science & Technology 2015 49 (4), 2577-2584 DOI: 10.1021/es505521w
dc.identifier0013-936X
dc.identifierhttps://hdl.handle.net/10495/33625
dc.identifier10.1021/es505521w
dc.identifier1520-5851
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229978
dc.descriptionABSTRACT: Effective domestic wastewater treatment is among our primary defenses against the dissemination of infectious waterborne disease. However, reducing the amount of energy used in treatment processes has become essential for the future. One low-energy treatment option is anaerobic–aerobic sequence (AAS) bioreactors, which use an anaerobic pretreatment step (e.g., anaerobic hybrid reactors) to reduce carbon levels, followed by some form of aerobic treatment. Although AAS is common in warm climates, it is not known how its compares to other treatment options relative to disease transmission, including its influence on antibiotic resistance (AR) in treated effluents. Here, we used metagenomic approaches to contrast the fate of antibiotic-resistant genes (ARG) in anaerobic, aerobic, and AAS bioreactors treating domestic wastewater. Five reactor configurations were monitored for 6 months, and treatment performance, energy use, and ARG abundance and diversity were compared in influents and effluents. AAS and aerobic reactors were superior to anaerobic units in reducing ARG-like sequence abundances, with effluent ARG levels of 29, 34, and 74 ppm (198 ppm influent), respectively. AAS and aerobic systems especially reduced aminoglycoside, tetracycline, and β-lactam ARG levels relative to anaerobic units, although 63 persistent ARG subtypes were detected in effluents from all systems (of 234 assessed). Sulfonamide and chloramphenicol ARG levels were largely unaffected by treatment, whereas a broad shift from target-specific ARGs to ARGs associated with multi-drug resistance was seen across influents and effluents. AAS reactors show promise for future applications because they can reduce more ARGs for less energy (32% less energy here), but all three treatment options have limitations and need further study.
dc.descriptionCOL0040402
dc.format8
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Chemical Society
dc.publisherDiagnóstico y Control de la Contaminación
dc.publisherWashington, Estados Unidos
dc.relationEnviron. Sci. Technol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectFarmacorresistencia Microbiana
dc.subjectDrug Resistance, Microbial
dc.subjectGenes Bacterianos
dc.subjectGenes, Bacterial
dc.subjectMetagenómica
dc.subjectMetagenomics
dc.subjectAguas del Alcantarillado - microbiología
dc.subjectSewage - microbiology
dc.subjectEliminación de Residuos Líquidos
dc.subjectWaste Disposal, Fluid
dc.subjectAguas Residuales - microbiología
dc.subjectWastewater - microbiology
dc.subjectContaminación Química del Agua
dc.subjectWater Pollution, Chemical
dc.subjectReactores Biológicos
dc.subjectBioreactors
dc.titleMetagenomics Shows That Low-Energy Anaerobic−Aerobic Treatment Reactors Reduce Antibiotic Resistance Gene Levels from Domestic Wastewater
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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