Neural Differentiation of Transplanted Neural Stem Cells in a Rat Model of Striatal Lacunar Infarctin: Ligth Electron Microscopic Observations

dc.creatorCardona Gómez, Gloria Patricia
dc.creatorMuñetón Gómez, Vilma Consuelo
dc.creatorDoncel Pérez, Ernesto
dc.creatorFernandez Fernandez, Ana Patricia
dc.creatorSerrano Masa, Julia
dc.creatorPozo Rodrigálvarez, Andrea
dc.creatorVellosillo Huerta, Lara
dc.creatorTaylor, Julian
dc.creatorNieto Sampedro, Manuel
dc.creatorMartínez Murillo, Ricardo
dc.date2023-05-25T22:37:10Z
dc.date2023-05-25T22:37:10Z
dc.date2012
dc.date.accessioned2024-04-23T17:49:46Z
dc.date.available2024-04-23T17:49:46Z
dc.identifierMuñetón-Gómez VC, Doncel-Pérez E, Fernandez AP, Serrano J, Pozo-Rodrigálvarez A, Vellosillo-Huerta L, Taylor JS, Cardona-Gómez GP, Nieto-Sampedro M, Martínez-Murillo R. Neural differentiation of transplanted neural stem cells in a rat model of striatal lacunar infarction: light and electron microscopic observations. Front Cell Neurosci. 2012 Aug 2;6:30. doi: 10.3389/fncel.2012.00030.
dc.identifierhttps://hdl.handle.net/10495/35115
dc.identifier10.3389/fncel.2012.00030.
dc.identifier1662-5102
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229945
dc.descriptionABSTRACT: The increased risk and prevalence of lacunar stroke and Parkinson’s disease (PD) makes the search for better experimental models an important requirement for translational research. In this study we assess ischemic damage of the nigrostriatal pathway in a model of lacunar stroke evoked by damaging the perforating arteries in the territory of the substantia nigra (SN) of the rat after stereotaxic administration of endothelin-1 (ET-1), a potent vasoconstrictor peptide. We hypothesized that transplantation of neural stem cells (NSCs) with the capacity of differentiating into diverse cell types such as neurons and glia, but with limited proliferation potential, would constitute an alternative and/or adjuvant therapy for lacunar stroke. These cells showed neuritogenic activity in vitro and a high potential for neural differentiation. Light and electron microscopy immunocytochemistry was used to characterize GFP-positive neurons derived from the transplants. 48 h after ET1 injection, we characterized an area of selective degeneration of dopaminergic neurons within the nigrostriatal pathway characterized with tissue necrosis and glial scar formation, with subsequent behavioral signs of Parkinsonism. Light microscopy showed that grafted cells within the striatal infarction zone differentiated with a high yield into mature glial cells (GFAP-positive) and neuron types present in the normal striatum. Electron microscopy revealed that NSCs-derived neurons integrated into the host circuitry establishing synaptic contacts, mostly of the asymmetric type. Astrocytes were closely associated with normal small-sized blood vessels in the area of infarct, suggesting a possible role in the regulation of the blood brain barrier and angiogenesis. Our results encourage the use of NSCs as a cell-replacement therapy for the treatment of human vascular Parkinsonism.
dc.descriptionCOL0010744
dc.format19
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.publisherGrupo de Neurociencias de Antioquia
dc.publisherLausana, Suiza
dc.relationFront. Cell. Neurosci.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectMicroscopía Electrónica
dc.subjectMicroscopy, Electron
dc.subjectImmunohistochemistry
dc.subjectInmunohistoquímica
dc.subjectNeural Stem Cells
dc.subjectCélulas-Madre Neurales
dc.subjectStroke
dc.subjectAccidente Cerebrovascular
dc.subjectParkinson Disease, Secondary
dc.subjectEnfermedad de Parkinson Secundaria
dc.subjectCorpus Striatum
dc.subjectCuerpo Estriado
dc.subjectDopamine Agents
dc.subjectDopaminérgicos
dc.titleNeural Differentiation of Transplanted Neural Stem Cells in a Rat Model of Striatal Lacunar Infarctin: Ligth Electron Microscopic Observations
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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