Genome Size and Ploidy of Paracoccidioides Brasiliensis Reveals a Haploid DNA Content : Flow Cytometry and GP43 Sequence Analysis

dc.creatorAlmeida Ramalho, Agostinho Joao
dc.creatorMatute González, Daniel Ricardo
dc.creatorCarmona, Jenifer Andrea
dc.creatorMartins, Margarida
dc.creatorTorres Gomez, Isaura Patricia
dc.creatorMcEwen Ochoa, Juan Guillermo
dc.creatorRestrepo Moreno, Angela
dc.creatorLudovico, Paula
dc.creatorLeão, Cecília
dc.creatorRodrigues, Fernando José Santos
dc.date2023-02-13T16:50:53Z
dc.date2023-02-13T16:50:53Z
dc.date2007
dc.date.accessioned2024-04-23T17:46:48Z
dc.date.available2024-04-23T17:46:48Z
dc.identifier1087-1845
dc.identifierhttps://hdl.handle.net/10495/33467
dc.identifier10.1016/j.fgb.2006.06.003
dc.identifier1096-0937
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S1087184506001186#!
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229879
dc.descriptionABSTRACT The aim of this study was to evaluate genome size and ploidy of the dimorphic pathogenic fungus Paracoccidioides brasiliensis. The cell cycle analysis of 10 P. brasiliensis isolates by flow cytometry (FCM) revealed a genome size ranging from 26.3 ± 0.1 Mb (26.9 ± 0.1 fg) to 35.5 ± 0.2 Mb (36.3 ± 0.2 fg) per uninucleated yeast cell. The DNA content of conidia from P. brasiliensis ATCC 60855—30.2 ± 0.8 Mb (30.9 ± 0.8 fg) —showed no significant differences with the yeast form, possibly excluding the occurrence of ploidy shift during morphogenesis. The ploidy of several P. brasiliensis isolates was assessed by comparing genome sizing by FCM with the previously described average haploid size obtained from electrophoretic karyotyping. The analysis of intra-individual variability of a highly polymorphic P. brasiliensis gene, GP43, indicated that only one allele seems to be present. Overall, the results showed that all analysed isolates presented a haploid, or at least aneuploid, DNA content and no association was detected between genome size/ploidy and the clinical-epidemiological features of the studied isolates. This work provides new knowledge on P. brasiliensis genetics/genomics, important for future research in basic cellular/molecular mechanisms and for the development/design of molecular techniques in this fungus.
dc.descriptionCOL0000962
dc.format7
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.publisherBiología Celular y Molecular CIB U. de A. U. del Rosario
dc.publisherOrlando, Estados Unidos
dc.relationFungal Genet. Biol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParacoccidioides
dc.subjectGenome Size
dc.subjectTamaño del Genoma
dc.subjectPloidies
dc.subjectPloidias
dc.titleGenome Size and Ploidy of Paracoccidioides Brasiliensis Reveals a Haploid DNA Content : Flow Cytometry and GP43 Sequence Analysis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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