Colombia
| info:eu-repo/semantics/article
Multitranscript analysis reveals an effect of 2-deoxy-d-glucose on gene expression linked to unfolded protein response and integrated stress response in primary human monocytes and monocyte-derived macrophages
| dc.creator | Urcuqui Inchima, Silvio | |
| dc.creator | Velilla Hernández, Paula Andrea | |
| dc.creator | Tamayo Molina, Yordi Sebastián | |
| dc.creator | Hernández Sarmiento, Lady Johana | |
| dc.date | 2023-06-15T18:10:26Z | |
| dc.date | 2023-06-15T18:10:26Z | |
| dc.date | 2023 | |
| dc.date.accessioned | 2024-04-23T17:44:22Z | |
| dc.date.available | 2024-04-23T17:44:22Z | |
| dc.identifier | Tamayo-Molina YS, Velilla PA, Hernández-Sarmiento LJ, Urcuqui-Inchima S. Multitranscript analysis reveals an effect of 2-deoxy-d-glucose on gene expression linked to unfolded protein response and integrated stress response in primary human monocytes and monocyte-derived macrophages. Biochim Biophys Acta Gen Subj. 2023 Jun 7;1867(9):130397. doi: 10.1016/j.bbagen.2023.130397. Epub ahead of print. PMID: 37290716. | |
| dc.identifier | 0304-4165 | |
| dc.identifier | https://hdl.handle.net/10495/35522 | |
| dc.identifier | 10.1016/j.bbagen.2023.130397 | |
| dc.identifier | 1872-8006 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9229825 | |
| dc.description | ABSTRACT: Background: Glycolytic inhibitor 2-deoxy-D-glucose (2-DG) binds to hexokinase in a non-competitive manner and phosphoglucose isomerase in a competitive manner, blocking the initial steps of the glycolytic pathway. Although 2-DG stimulates endoplasmic reticulum (ER) stress, activating the unfolded protein response to restore protein homeostasis, it is unclear which ER stress-related genes are modulated in response to 2-DG treatment in human primary cells. Here, we aimed to determine whether the treatment of monocytes and monocyte-derived macrophages (MDMs) with 2-DG leads to a transcriptional profile specific to ER stress. Methods: We performed bioinformatics analysis to identify differentially expressed genes (DEGs) in previously reported RNA-seq datasets of 2-DG treated cells. RT-qPCR was performed to verify the sequencing data on cultured MDMs. Results: A total of 95 common DEGs were found by transcriptional analysis of monocytes and MDMs treated with 2-DG. Among these, 74 were up-regulated and 21 were down-regulated. Multitranscript analysis showed that DEGs are linked to integrated stress response (GRP78/BiP, PERK, ATF4, CHOP, GADD34, IRE1α, XBP1, SESN2, ASNS, PHGDH), hexosamine biosynthetic pathway (GFAT1, GNA1, PGM3, UAP1), and mannose metabolism (GMPPA and GMPPB). Conclusions: Results reveal that 2-DG triggers a gene expression program that might be involved in restoring protein homeostasis in primary cells. General significance: 2-DG is known to inhibit glycolysis and induce ER stress; however, its effect on gene expression in primary cells is not well understood. This work shows that 2-DG is a stress inducer shifting the metabolic state of monocytes and macrophages | |
| dc.description | COL0012444 | |
| dc.format | 9 | |
| dc.format | application/pdf | |
| dc.format | application/pdf | |
| dc.format | application/vnd.openxmlformats-officedocument.wordprocessingml.document | |
| dc.language | eng | |
| dc.publisher | Elsevier | |
| dc.publisher | Inmunovirología | |
| dc.publisher | Ámsterdam, Países Bajos | |
| dc.relation | Biochim. Biophys. Acta, Gen. Subj. | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | |
| dc.rights | http://purl.org/coar/access_right/c_abf2 | |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Desoxiglucosa | |
| dc.subject | Deoxyglucose | |
| dc.subject | Estrés del Retículo Endoplásmico | |
| dc.subject | Endoplasmic Reticulum Stress | |
| dc.subject | Respuesta de Proteína Desplegada | |
| dc.subject | Unfolded Protein Response | |
| dc.subject | Glicosilación | |
| dc.subject | Glycosylation | |
| dc.subject | Células | |
| dc.subject | Cells | |
| dc.title | Multitranscript analysis reveals an effect of 2-deoxy-d-glucose on gene expression linked to unfolded protein response and integrated stress response in primary human monocytes and monocyte-derived macrophages | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
| dc.type | http://purl.org/coar/resource_type/c_2df8fbb1 | |
| dc.type | https://purl.org/redcol/resource_type/ART | |
| dc.type | Artículo de investigación |