Human cells and cell membrane molecular models are affected in vitro by the nonsteroidal anti-inflammatory drug ibuprofen

dc.creatorManrique Moreno, Marcela María
dc.creatorVillena, Fernando
dc.creatorP. Sotomayor, Carlos
dc.creatorEdwards, Ana M.
dc.creatorMuñoz, Marcelo A.
dc.creatorGaridel, Patrick
dc.creatorSuwalsky, Mario
dc.date2023-04-06T23:33:25Z
dc.date2023-04-06T23:33:25Z
dc.date2011
dc.date.accessioned2024-04-23T14:22:19Z
dc.date.available2024-04-23T14:22:19Z
dc.identifierManrique-Moreno M, Villena F, Sotomayor CP, Edwards AM, Muñoz MA, Garidel P, Suwalsky M. Human cells and cell membrane molecular models are affected in vitro by the nonsteroidal anti-inflammatory drug ibuprofen. Biochim Biophys Acta. 2011 Nov;1808(11):2656-64. doi: 10.1016/j.bbamem.2011.07.005.
dc.identifier0005-2736
dc.identifierhttps://hdl.handle.net/10495/34485
dc.identifierdoi:10.1016/j.bbamem.2011.07.005
dc.identifier1879-2642
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229608
dc.descriptionABSTRACT: This report presents evidence that ibuprofen interacts with red cell membranes as follows: a) in scanning electron microscopy (SEM) studies on human erythrocytes induced shape changes at a concentration as low as 10μM; b) in isolated unsealed human erythrocyte membranes (IUM) induced mild increase in the water content or in their molecular dynamics at the hydrophobic-hydrophilic interphase, while a corresponding ordering decrease at the deep phospholipids acyl chain level; c) at physiological temperature (37°C), 300μM ibuprofen induced a significant increase in the generalized polarization (GP) of dimyristoylphosphatidylcholine (DMPC) large unilamellar vesicles (LUV), an indication that ibuprofen molecules locate in the head polar group region of DMPC; d) X-ray diffraction studies showed that ibuprofen concentrations≥300μM induced increasing structural perturbation to DMPC bilayers; e) differential scanning calorimetry (DSC) data showed that ibuprofen was able to alter the cooperativity of DMPC phase transition in a concentration-dependent manner, to destabilize the gel phase and that ibuprofen did not significantly perturb the organization of the lipid hydrocarbon chains. Additionally, the effect on the viability of both human promyelocytic leukemia HL-60 and human cervical carcinoma HeLa cells was studied.
dc.descriptionCOL0156275
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.publisherGrupo de Bioquímica Estructural de Macromoléculas
dc.publisherÁmsterdam, Países Bajos
dc.relationBiochim. Biophys. Acta. Biomembr.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAnti-Inflammatory Agents, Non-Steroidal
dc.subjectAntiinflamatorios no Esteroideos
dc.subjectCell membrane
dc.subjectMembrana Celular
dc.subjectCalorimetry, Differential Scanning
dc.subjectRastreo Diferencial de Calorimetría
dc.subjectIbuprofen
dc.subjectIbuprofeno
dc.subjectCell Line, Tumor
dc.subjectLínea Celular Tumoral
dc.subjectDimyristoylphosphatidylcholine
dc.subjectDimiristoilfosfatidilcolina
dc.subjectErythrocytes - drug effects
dc.subjectEritrocitos - efectos de los fármacos
dc.subjectMicroscopy, Electron, Scanning
dc.subjectMicroscopía Electrónica de Rastreo
dc.subjectModels, Molecular
dc.subjectModelos Moleculares
dc.subjectUnilamellar Liposomes
dc.subjectLiposomas Unilamelares
dc.subjectX-Ray Diffraction
dc.subjectDifracción de Rayos X
dc.titleHuman cells and cell membrane molecular models are affected in vitro by the nonsteroidal anti-inflammatory drug ibuprofen
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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