Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants

dc.creatorPezzotti, Gianni
dc.creatorScognamiglio, Viviana
dc.creatorDimova Lambreva, Maya
dc.creatorTibuzzi, Arianna
dc.creatorJohanningmeier, U.
dc.creatorRaffi, D.
dc.creatorRea, Giuseppina
dc.creatorGiardi, Maria Teresa
dc.date2023-04-04T12:48:16Z
dc.date2023-04-04T12:48:16Z
dc.date2009
dc.date.accessioned2024-04-23T14:21:20Z
dc.date.available2024-04-23T14:21:20Z
dc.identifierScognamiglio, Viviana & Raffi, D & Lambreva, Maya & Rea, Giuseppina & Tibuzzi, Arianna & Pezzotti, Gianni & Johanningmeier, U & Giardi, Maria. (2009). Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants. Analytical and bioanalytical chemistry. 394. 1081-7. 10.1007/s00216-009-2668-1.
dc.identifier1618-2642
dc.identifierhttps://hdl.handle.net/10495/34441
dc.identifier10.1007/s00216-009-2668-1
dc.identifier1618-2650
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229575
dc.descriptionABSTRACT: The unicellular green alga Chlamydomonas reinhardtii is employed here for the setup of a biosensor demonstrator based on multibiomediators for the detection of herbicides. The detection is based on the activity of photosystem II, the multienzymatic chlorophyll–protein complex located in the thylakoid membrane that catalyzes the light-dependent photosynthetic primary charge separation and the electron transfer chain in cyanobacteria, algae, and higher plants. Several C. reinhardtii mutants modified on the D1 photosystem II protein are generated by site-directed mutagenesis and experimentally tested for the development of a biosensor revealing the modification of the fluorescence parameter (1−VJ) in the presence of herbicides. The A250R, A250L, A251C, and I163N mutants are highly sensitive to the urea and triazine herbicide classes; the newly generated F255N mutant is shown to be especially resistant to the class of urea. It follows that the response of the multibiomediators is associated to a particular herbicide subclass and can be useful to monitor several species of pollutants
dc.descriptionCOL0010477
dc.format6
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.publisherGrupo de Manejo Eficiente de la Energía (GIMEL)
dc.publisherHeidelberg, Alemania
dc.relationAnal. Bioanal. Chem.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChlamydomonas reinhardtii
dc.subjectTécnicas Biosensibles
dc.subjectBiosensing Techniques
dc.subjectMutagénesis Sitio-Dirigida
dc.subjectMutagenesis, Site-Directed
dc.subjectContaminantes Ambientales
dc.subjectEnvironmental Pollutants
dc.subjectFluorescencia
dc.subjectFluorescence
dc.subjectVariación Genética
dc.subjectGenetic Variation
dc.subjectHerbicidas - análisis
dc.subjectHerbicides - analysis
dc.subjectComplejo de Proteína del Fotosistema II
dc.subjectPhotosystem II Protein Complex
dc.subjectFactores de Tiempo
dc.subjectTime Factors
dc.subjectSensibilidad y Especificidad
dc.subjectSensitivity and Specificity
dc.titleChlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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