Vitamin D modulates inflammatory response of DENV-2-infected macrophages by inhibiting the expression of inflammatory-liked miRNAs

dc.creatorCastillo Ramírez, Jorge Andrés
dc.creatorUrcuqui Inchima, Silvio
dc.date2023-05-18T17:09:34Z
dc.date2023-05-18T17:09:34Z
dc.date2023
dc.date.accessioned2024-04-23T14:20:32Z
dc.date.available2024-04-23T14:20:32Z
dc.identifierCastillo JA, Urcuqui-Inchima S. Vitamin D modulates inflammatory response of DENV-2-infected macrophages by inhibiting the expression of inflammatory-liked miRNAs. Pathog Glob Health. 2023 Mar;117(2):167-180. doi: 10.1080/20477724.2022.2101840.
dc.identifier2047-7724
dc.identifierhttps://hdl.handle.net/10495/35044
dc.identifier10.1080/20477724.2022.2101840
dc.identifier2047-7732
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229554
dc.descriptionABSTRACT: Dengue disease caused by dengue virus (DENV) infection is the most common vector-borne viral disease worldwide. Currently, no treatment is available to fight dengue symptoms. We and others have demonstrated the antiviral and immunomodulatory properties of VitD3 as a possible therapy for DENV infection. MicroRNAs (miRNAs) are small non-coding RNAs responsible for the regulation of cell processes including antiviral defense. Previous transcriptomic analysis showed that VitD3 regulates the expression of genes involved in stress and immune response by inducing specific miRNAs. Here, we focus on the effects of VitD3 supplementation in the regulation of the expression of inflammatory-liked miR-182-5p, miR-130a-3p, miR125b-5p, miR146a-5p, and miR-155-5p during DENV-2 infection of monocyte-derived macrophages (MDMs). Further, we evaluated the effects of inhibition of these miRNAs in the innate immune response. Our results showed that supplementation with VitD3 differentially regulated the expression of these inflammatory miRNAs. We also observed that inhibition of miR-182-5p, miR-130a-3p, miR-125b-5p, and miR-155-5p, led to decreased production of TNF-α and TLR9 expression, while increased the expression of SOCS-1, IFN-β, and OAS1, without affecting DENV replication. By contrast, over-expression of miR-182-5p, miR-130a-3p, miR-125b-5p, and miR-155-5p significantly decreased DENV-2 infection rates and also DENV-2 replication in MDMs. Our results suggest that VitD3 immunomodulatory effects involve regulation of inflammation-linked miRNAs expression, which might play a key role in the inflammatory response during DENV infection.
dc.descriptionCOL0012444
dc.format14
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherTaylor & Francis
dc.publisherInmunovirología
dc.publisherLondres, Inglaterra
dc.relationPathog. Glob. Health.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectVirus del Dengue
dc.subjectDengue Virus
dc.subjectDengue - inmunología
dc.subjectDengue - immunology
dc.subjectRegulación de la Expresión Génica
dc.subjectGene Expression Regulation
dc.subjectMacrófagos
dc.subjectMacrophages
dc.subjectMicroARNs - genética
dc.subjectMicroRNAs - genetics
dc.subjectReplicación Viral
dc.subjectVirus Replication
dc.subjectVitamina D - farmacología
dc.subjectVitamin D - pharmacology
dc.subjectInmunidad Innata
dc.subjectImmunity, Innate
dc.titleVitamin D modulates inflammatory response of DENV-2-infected macrophages by inhibiting the expression of inflammatory-liked miRNAs
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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