Pegylation of poly([gamma]-benzyl-L-glutamate) nanoparticles is efficient for avoiding mononuclear phagocyte system capture in rats

dc.creatorSegura Sánchez, Freimar
dc.creatorOzcan, Ipek
dc.creatorBouchemal, Kawthar
dc.creatorSezak, Murat
dc.creatorOzer, Ozgen
dc.creatorGuneri, Tamer
dc.creatorPonchel, Gilles
dc.date2023-06-30T15:28:41Z
dc.date2023-06-30T15:28:41Z
dc.date2010
dc.date.accessioned2024-04-23T14:19:39Z
dc.date.available2024-04-23T14:19:39Z
dc.identifierOzcan I, Segura-Sánchez F, Bouchemal K, Sezak M, Ozer O, Güneri T, Ponchel G. Pegylation of poly(γ-benzyl-L-glutamate) nanoparticles is efficient for avoiding mononuclear phagocyte system capture in rats. Int J Nanomedicine. 2010 Dec 8;5:1103-11. doi: 10.2147/IJN.S15493.
dc.identifier1176-9114
dc.identifierhttps://hdl.handle.net/10495/35730
dc.identifier10.2147/IJN.S15493
dc.identifier1178-2013
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229529
dc.descriptionABSTRACT: Poly(γ-benzyl-L-glutamate) (PBLG) derivatives are synthetic polypeptides for preparing nanoparticles with well controlled surface properties. The aim of this paper was to investigate the biodistribution of pegylated PBLG in rats. For this purpose, nanoparticles were prepared by a nanoprecipitation method using mixtures of different PBLG derivates, including a pegylated derivate to avoid mononuclear phagocyte system uptake. The morphology, size distribution, and surface charge of the nanoparticles were investigated as a function of the amount of polymer employed for the preparation. Moderately polydispersed nanoparticles (polydispersity index less than 0.2) were obtained. Their size increased with polymer concentration. The zeta potential values were negative whatever the formulations. The availability of polyethylene glycol chains on the nanoparticles’ surface was confirmed by measuring the decrease in bovine serum albumin adsorption. For in vivo distribution studies, pegylated and nonpegylated nanoparticles were prepared with polymer mixtures containing PBLG-fluorescein isothiocyanate and imaged by fluorescence microscopy to measure their accumulation in liver and spleen tissues of rats after intravenous administration. Injection of stealth formulations resulted in negligible fluorescence in liver and spleen compared with nonpegylated formulations, which suggests that these nanoparticles are promising candidates as a stealth-type long-circulating drug carrier system and could be useful for active targeting of drugs while reducing systemic side effects.
dc.descriptionCOL0065152
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherDove Medical Press
dc.publisherBIOPOLIMER
dc.publisherAuckland, Nueva Zelanda
dc.relationInt. J. Nanomedicine.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAnálisis de Varianza
dc.subjectAnalysis of Variance
dc.subjectSistemas de Liberación de Medicamentos
dc.subjectDrug Delivery Systems
dc.subjectFluoresceína-5-Isotiocianato
dc.subjectFluorescein-5-isothiocyanate
dc.subjectHígado - Química
dc.subjectLiver - Chemistry
dc.subjectEspectroscopía de Resonancia Magnética
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectMicroscopía Electrónica de Transmisión
dc.subjectMicroscopy, Electron, Transmission
dc.subjectMicroscopía Fluorescente
dc.subjectMicroscopy, Fluorescence
dc.subjectPeso Molecular
dc.subjectMolecular Weight
dc.subjectNanopartículas - Química
dc.subjectNanoparticles - Chemistry
dc.subjectFagocitosis
dc.subjectPhagocytosis
dc.subjectPolietilenglicoles - Química
dc.subjectPolyethylene Glycols - Chemistry
dc.subjectÁcido Poliglutámico
dc.subjectPolyglutamic Acid
dc.subjectRatas
dc.subjectRats
dc.subjectAlbúmina Sérica Bovina
dc.subjectSerum Albumin, Bovine
dc.subjectBazo - Química
dc.subjectSpleen - Chemistry
dc.subjectRelación Estructura-Actividad
dc.subjectStructure-Activity Relationship
dc.subjectDistribución Tisular
dc.subjectTissue Distribution
dc.titlePegylation of poly([gamma]-benzyl-L-glutamate) nanoparticles is efficient for avoiding mononuclear phagocyte system capture in rats
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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