dc.creator | Zapata Montoya, Jose Edgar | |
dc.creator | Gómez Sampedro, Leidy Johanna | |
dc.creator | Gómez Grimaldos, Nathalia Andrea | |
dc.creator | López García, Gabriel | |
dc.creator | Cilla, Antonio | |
dc.creator | Alegría, Amparo | |
dc.date | 2023-05-29T20:17:25Z | |
dc.date | 2023-05-29T20:17:25Z | |
dc.date | 2020 | |
dc.date.accessioned | 2024-04-23T14:13:22Z | |
dc.date.available | 2024-04-23T14:13:22Z | |
dc.identifier | Gómez, L.J.; Gómez, N.A.; Zapata, J.E.; López-García, G.; Cilla, A.; Alegría, A. Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability. Foods 2020, 9, 883. https://doi.org/10.3390/foods9070883 | |
dc.identifier | https://hdl.handle.net/10495/35149 | |
dc.identifier | 10.3390/foods9070883 | |
dc.identifier | 2304-8158 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9229274 | |
dc.description | ABSTRACT: Iron deficiencies continue to cause significant health problems in vulnerable populations. A good strategy to combat mineral deficiency includes fortification with iron-binding peptides. This research aims to determine the optimal conditions to hydrolyze red tilapia viscera (RTV) using Alcalase 2.4 L and recovery of iron-binding protein hydrolysate. The result showed that under the optimal hydrolysis condition including pH 10, 60 ◦C, E/S ratio of 0.306 U/g protein, and substrate concentration of 8 g protein/L, the obtained hydrolysate with 42.5% degree of hydrolysis (RTVH-B), displayed the maximal iron-binding capacity of 67.1 ± 1.9%. Peptide fractionation was performed using ultrafiltration and the <1 kDa fraction (FRTVH-V) expressed the highest iron-binding capacity of 95.8 ± 1.5%. Iron content of RTVH-B and its fraction was assessed, whereas iron uptake was measured indirectly as ferritin synthesis in a Caco-2 cell model and the result showed that bioavailability of bound minerals from protein complexes was significantly higher (p < 0.05) than iron salt in its free form, increased 4.7 times for the Fe2+–RTVH-B complex. This research suggests a potential application of RTVH-B as dietary supplements to improve iron absorption. | |
dc.description | COL0010771 | |
dc.format | 16 | |
dc.format | application/pdf | |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | MDPI | |
dc.publisher | Grupo de Nutrición y Tecnología de Alimentos | |
dc.publisher | Basilea, Suiza | |
dc.relation | Foods | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights | http://creativecommons.org/licenses/by/2.5/co/ | |
dc.rights | http://purl.org/coar/access_right/c_abf2 | |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Células CACO-2 | |
dc.subject | Caco-2 Cells | |
dc.subject | Tilapia | |
dc.subject | Proteínas Hierro-Azufre | |
dc.subject | Iron-Sulfur Proteins | |
dc.subject | Hidrólisis | |
dc.subject | Hydrolysis | |
dc.subject | Subtilisinas | |
dc.subject | Subtilisins | |
dc.subject | Disponibilidad Biológica | |
dc.subject | Biological Availability | |
dc.subject | Hierro de la Dieta | |
dc.subject | Iron, Dietary | |
dc.title | Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.type | https://purl.org/redcol/resource_type/ART | |
dc.type | Artículo de investigación | |