Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

dc.creatorRobledo Restrepo, Sara María
dc.creatorCarrillo Bonilla, Lina María
dc.creatorRodríguez, Berardo de Jesús
dc.creatorDaza Figueredo, Juan Alejandro
dc.creatorRestrepo Agudelo, Adriana María
dc.creatorMuñoz Herrera, Diana Lorena
dc.creatorTobón Peña, Jairo Andrés
dc.creatorMurillo Arroyave, Javier Darío
dc.creatorLópez Arias, Anderson
dc.creatorRíos Úsuga, Carolina
dc.creatorMesa Castro, Carol Vanesa
dc.creatorYulieth Alexandra, Upegui Zapata
dc.creatorValencia Tobón, Alejandro
dc.creatorMondragón Shem, Karina
dc.creatorVélez Bernal, Iván Darío
dc.date2023-02-06T23:52:38Z
dc.date2023-02-06T23:52:38Z
dc.date2012
dc.date.accessioned2024-04-23T14:09:47Z
dc.date.available2024-04-23T14:09:47Z
dc.identifierRobledo SM, Carrillo LM, Daza A, Restrepo AM, Muñoz DL, Tobón J, Murillo JD, López A, Ríos C, Mesa CV, Upegui YA, Valencia-Tobón A, Mondragón-Shem K, Rodríguez B, Vélez ID. Cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs. J Vis Exp. 2012 Apr 21;(62):3533. doi: 10.3791/3533.
dc.identifierhttps://hdl.handle.net/10495/33383
dc.identifier10.3791/3533
dc.identifier1940-087X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9229145
dc.descriptionABSTRACT: Traditionally, hamsters are experimentally inoculated in the snout or the footpad. However in these sites an ulcer not always occurs, measurement of lesion size is a hard procedure and animals show difficulty to eat, breathe and move because of the lesion. In order to optimize the hamster model for cutaneous leishmaniasis, young adult male and female golden hamsters (Mesocricetus auratus) were injected intradermally at the dorsal skin with 1 to 1.5 x l07 promastigotes of Leishmania species and progression of subsequent lesions were evaluated for up to 16 weeks post infection. The golden hamster was selected because it is considered the adequate bio-model to evaluate drugs against Leishmania as they are susceptible to infection by different species. Cutaneous infection of hamsters results in chronic but controlled lesions, and a clinical evolution with signs similar to those observed in humans. Therefore, the establishment of the extent of infection by measuring the size of the lesion according to the area of indurations and ulcers is feasible. This approach has proven its versatility and easy management during inoculation, follow up and characterization of typical lesions (ulcers), application of treatments through different ways and obtaining of clinical samples after different treatments. By using this method the quality of animal life regarding locomotion, search for food and water, play and social activities is also preserved.
dc.descriptionCOL0015099
dc.descriptionCOL0092964
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMyJove Corporation
dc.publisherGrupo de Investigación en Patobiología – QUIRÓN
dc.publisherPrograma de Estudio y Control de Enfermedades Tropicales (PECET)
dc.publisherBoston, Estados Unidos
dc.relationJ. Vis. Exp.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntiprotozoarios
dc.subjectAntiprotozoal Agents
dc.subjectLeishmaniasis Cutánea
dc.subjectLeishmaniasis, Cutaneous
dc.subjectCricetinae
dc.subjectModelos Animales de Enfermedad
dc.subjectDisease Models, Animal
dc.subjectLeishmania - crecimiento y desarrollo
dc.subjectLeishmania - growth and development
dc.subjectEvaluación Preclínica de Medicamentos
dc.subjectDrug Evaluation, Preclinical
dc.subjectMesocricetus
dc.titleCutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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